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Osteogenic differentiation of ES cell-derived EBs mediated by embedded BMP-2 and TGF-beta-1 in a polyelectrolyte multilayer film

Published online by Cambridge University Press:  01 February 2011

Mjahed Hajare ,
Cortial Delphine ,
Haikel Youssef ,
Dierich Andree ,
Voegel Jean-Claude  and
Benkirane Jessel nadia
Mjahed Hajare
Affiliation:
INSERM, Biomaterials, INSERM UMR 595, Faculté de médecine, 11 rue Humann,, Strasbourg, 67084, France, 00 33 3 90 24 33 76, 00 33 3 90 24 33 79
Cortial Delphine
Affiliation:
[email protected], INSERM, Biomaterials, UMR 595, Faculté de médecine, 11 rue Humann, Strasbourg, 67084, France
Haikel Youssef
Affiliation:
[email protected], INSERM, Faculté de Chirurgie Dentaire,Université Louis Pasteur, Strasbourg, 67000, France
Dierich Andree
Affiliation:
[email protected], CNRS, ES, IGBMC, BP 10142, Strasbourg, France., Strasbourg, 67084, France
Voegel Jean-Claude
Affiliation:
voegel@medecine;u-strasbg.fr, INSERM, Biomaterials, UMR 595, Faculté de médecine, 11 rue Humann, Strasbourg, 67084, France
Benkirane Jessel nadia
Affiliation:
[email protected], INSERM, Biomaterials, UMR 595, Faculté de médecine, 11 rue Humann, Strasbourg, 67084, France
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Abstract

In recent years, considerable effort has been devoted to the design and controlled fabrication of structured materials with functional properties. The layer by layer buildup of polyelectrolyte multilayer films (PEM films) from oppositely charged polyelectrolytes1 offers new opportunities for the preparation of functionalized biomaterial coatings. This technique allows the preparation of supramolecular nano-architectures exhibiting specific properties in terms of control of cell activation and may also play a role in the development of local drug delivery systems. Peptides, proteins or DNA, chemically bound to polyelectrolytes, adsorbed or embedded in PEM films, have been shown to retain their biological activities. Recently, tissue engineering has merged with stem cell technology with interest to develop new sources of transplantable material for injury or disease treatment. Eminently interesting, are bone and joint injuries disorders because of the low self-regenerating capacity of the matrix secreting cells. We present here for the first time that embedded BMP-2 and TGFβ1 in a multilayered polyelectrolyte film can drive embryonic stem cells to the cartilage or bone differentiation depending on supplementary co-factors. We selected a model system made from layer by layer poly-ℓ-glutamic acid (PℓGA) and poly-ℓ-lysine succinylated (PℓLs) films into which BMP-2 and TGFβ1 have been embedded. Our results demonstrate clearly that we are able to induce osteogenesis in embryonic stem cells mediated by growth factors embedded in a polyelectrolyte multilayer film.

Keywords

tissuebiomaterialbone
Type
Research Article
Information
MRS Online Proceedings Library (OPL) , Volume 950: Symposium D – Biosurfaces and Biointerfaces , 2006 , 0950-D10-04
Copyright
Copyright © Materials Research Society 2007

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