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Organic-inorganic materials to immobilize biological complexes

Published online by Cambridge University Press:  17 December 2012

L. Valdez-Castro ,
L. Méndez-Amaro ,
R. Mendoza-Serna ,
M. L. Ramírez-Castillo  and
Jorge E Ibarra
L. Valdez-Castro
Affiliation:
Departamento de Ingeniería en Biotecnología, Universidad Politécnica de Puebla, Tercer Carril del Ejido Serrano S/N, San Mateo Cuanalá, Juan C. Bonilla, Puebla, México.
L. Méndez-Amaro
Affiliation:
Departamento de Ingeniería en Biotecnología, Universidad Politécnica de Puebla, Tercer Carril del Ejido Serrano S/N, San Mateo Cuanalá, Juan C. Bonilla, Puebla, México.
R. Mendoza-Serna
Affiliation:
Carrera de Ingeniería Química, Facultad de Estudios Superiores Zaragoza, Universidad Nacional Autónoma de México, Batalla 5 de Mayo S/N, Colonia Ejército de Oriente, Iztapalapa, México.
M. L. Ramírez-Castillo
Affiliation:
Departamento de Ingeniería en Biotecnología, Universidad Politécnica de Puebla, Tercer Carril del Ejido Serrano S/N, San Mateo Cuanalá, Juan C. Bonilla, Puebla, México.
Jorge E Ibarra
Affiliation:
Departamento Biotecnología y Bioquímica, CINVESTAV-IPN, Irapuato, Gto., México.
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Abstract

In this paper the spore-crystal complex of Bacillus thuringiensis var. israelensis (Bti) was immobilized by the sol-gel process in a hybrid polymer using as precursors the inorganic tetraethyl orthosilicate (TEOS) and the organic Polydimethylsiloxane (PDMS); in order to combine the advantages of both materials in a hybrid matrix to improve aspects such as the thermal stability, the hydrophobic properties and the porosity. Bti produces different crystals during sporulation phase; these are of protein nature and are used as bio-insecticides. It is important to mention that the insecticide attack is specific to the mosquito larva that causes dengue and black flies. The samples were characterized to ensure viability by performing growth kinetics with fermentations immersed in a flask, this microbial growth was monitored by dry weight, glucose consumption and characterized by Fourier Transform Infrared Spectroscopy (FTIR) to observe the interaction of materials with spore-crystal complex.

Keywords

BiologicalPolymer CompoundInfrared (IR) spectroscopy
Type
Articles
Information
MRS Online Proceedings Library (OPL) , Volume 1480: Symposium S – Novel Characterization Methods for Biological Systems , 2012 , imrc12-1480-s2b-p016
Copyright
Copyright © Materials Research Society 2012 

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References

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