Hostname: page-component-586b7cd67f-r5fsc Total loading time: 0 Render date: 2024-11-29T06:26:01.138Z Has data issue: false hasContentIssue false
  • English
  • Français

One- and Two-Particle Microrheology in Entangled Solutions of fd Virus

Published online by Cambridge University Press:  17 March 2011

Karim M. Addas ,
Alex J. Levine ,
Jay X. Tang  and
Christoph F. Schmidt
Karim M. Addas
Affiliation:
Department of Physics & Indiana Molecular Biology Institute, Indiana University, 727 East Third St., Bloomington, IN 47405, U.S.A.
Alex J. Levine
Affiliation:
Department of Chemical Engineering and Materials Research Laboratory, University of California, Santa Barbara, CA 93106-4030, U.S.A.
Jay X. Tang
Affiliation:
Department of Physics & Indiana Molecular Biology Institute, Indiana University, 727 East Third St., Bloomington, IN 47405, U.S.A.
Christoph F. Schmidt
Affiliation:
Department of Physics of Complex Systems, Vrije Universiteit Amsterdam, De Boelelaan 1081, 1081HV Amsterdam, The Netherlands
Get access

Abstract

We have used one- and two-particle microrheology, employing μm-sized beads and laser interferometric displacement detection, to study the rheological properties of entangled solutions of the filamentous fd virus. Thermal fluctuations of the embedded probes were measured and viscoelastic parameters of the embedding medium were derived. In two-particle microrheology the correlated motions of two identical particles separated by a varying distance in the medium are analyzed, which can avoid biased results due to surface-depletion effects near the probes.

Type
Research Article
Information
MRS Online Proceedings Library (OPL) , Volume 711: Symposia FF/GG/HH – Advanced Biomaterials--Characterization, Tissue Engineering and Complexity , 2001 , FF4.8.1
Copyright
Copyright © Materials Research Society 2002

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

REFERENCES

1. Larson, R. G., The structure and rheology of complex fluids (Oxford University Press, Oxford, 1998).Google Scholar
2. MacKintosh, F. C. and Schmidt, C. F., Current Opinion in Colloid & Interface Science 4, 300 (1999).Google Scholar
3. Schnurr, B., Gittes, F., MacKintosh, F. C., and Schmidt, C. F., Macromolecules 30, 7781 (1997).Google Scholar
4. Gittes, F., Schnurr, B., Olmsted, P. D., MacKintosh, F. C., and Schmidt, C. F., Physical Review Letters 79, 3286 (1997).Google Scholar
5. Crocker, J. C., Valentine, M. T., Weeks, E. R., Gisler, T., Kaplan, P. D., Yodh, A. G., and Weitz, D. A., Physical Review Letters 85, 888 (2000).Google Scholar
6. Levine, A. J. and Lubensky, T. C., Physical Review Letters 85, 1774 (2000).Google Scholar
7. Levine, A. J. and Lubensky, T. C., accepted in Phys. Rev. E, Preprint on condmat/0104132, (2002).Google Scholar
8. Makowski, L., in The Structures of Biological Macromolecules and Assemblies, Vol. 1, The Viruses, edited by McPherson, A. and Jurnak, F. (John Wiley and Sons, New York, 1984), p. 203.Google Scholar
9. Chaikin, P. M. and Lubensky, T. C., Principles of Condensed Matter Physics (Cambridge University Press, Cambridge, New York, 1995).Google Scholar
10. Mason, T. G. and Weitz, D. A., Physical Review Letters 74, 1250 (1995).Google Scholar
11. Sambrook, J., Fritsch, E. F., and Maniatis, T., Molecular Cloning: A Laboratory Manual (Cold Spring Harbor Laboratory Press, Cold Spring Harbor, 1989).Google Scholar
12. Payment, P. and Trudel, M., Methods and techniques in virology (Marcel Dekker, New York, 1993).Google Scholar
13. Allersma, M. W., Gittes, F., deCastro, M. J., Stewart, R. J., and Schmidt, C. F., Biophysical Journal 74, 1074 (1998).Google Scholar
14. Gittes, F. and Schmidt, C. F., Optics Letters 23, 7 (1998).Google Scholar
15. Ashkin, A., Proc Natl Acad Sci U S A 94, 4853 (1997).Google Scholar
16. Schmidt, F. G., Hinner, B., Sackmann, E., and Tang, J. X., Physical Review E 62, 5509 (2000).Google Scholar