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On-chip Detection of Chemiluminescent Biomolecules Using an Integrated Thin Film Silicon Photodiode

Published online by Cambridge University Press:  01 February 2011

A. Pereira ,
A. Pimentel ,
V. Chu ,
D. M. F. Prazeres  and
J. P. Conde
A. Pereira
Affiliation:
[email protected], INESC-MN, -, Rua Alves Redol, 9, Lisbon, 1000-029, Portugal
A. Pimentel
Affiliation:
[email protected], INESC-MN, Rua Alves Redol, 9, Lisbon, 1000-029, Portugal
V. Chu
Affiliation:
[email protected], INESC-MN, Rua Alves Redol, 9, Lisbon, 1000-029, Portugal
D. M. F. Prazeres
Affiliation:
[email protected], Instituto Superior Técnico, Centro de Engenharia Biológica e Química, Lisbon, 1049-001, Portugal
J. P. Conde
Affiliation:
[email protected], INESC-MN, Rua Alves Redol, 9, Lisbon, 1000-029, Portugal
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Abstract

A hydrogenated amorphous silicon photodetector on a glass substrate is used for the integrated, real-time detection of chemiluminescent reactions in solution. The light emission at 425 nm resulting from the horseradish peroxidase-catalyzed oxidation of luminol is detected by a p-i-n amorphous silicon photodiode operated at zero bias. The limit of detection of the present device is in the range of nanomole of horseradish peroxidase per liter.

Keywords

sensoroptoelectronicbiological
Type
Research Article
Information
MRS Online Proceedings Library (OPL) , Volume 1004: Symposium P – Materials and Strategies for Lab-on-a-Chip-Biological Analysis, Microfactories, and Fluidic Assembly of Nanostructures , 2007 , 1004-P04-10
Copyright
Copyright © Materials Research Society 2007

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References

1. Fixe, F., Chu, V., Prazeres, D. M. F. and Conde, J. P.. Nucleic Acids Research 32, e70 (2004).Google Scholar
2. Thorpe, G. H. G., Kricka, L. J., Moseley, S. B., and Whitehead, T. P.. Clinical Chemistry. 31(8), 1335 (1985).Google Scholar
3. Yakovleva, J., Lobanova, R. D. A., Bengtsson, M., Eremin, S., Laurell, T. and Emnéus, J.., Anal. Chem. 74(13), 2994 (2002).Google Scholar
4. Davidsson, R., Genin, F., Bengtsson, M., Laurell, T., and Emnéus, J, Lab Chip, 4(5), 481 (2004).Google Scholar
5. Mallard, F., Marchand, G., Ginot, F., and Campagnolo, R., Biosens. Bioelectron, 20(9), 1813 (2005).Google Scholar
6. Lu, U., Hu, B. C.-P-, Shih, Y.-C., Yang, Y.-S., Wu, C.-Yu, Yuan, C.-J., Ker, M.-D., Wu, T.-K., Li, Y.-K., Hsieh, Y.-Z., Hsu, W., and Lin, C.-T., IEEE Sensors Journal, 3(3), 310–316 (2003).Google Scholar
7. Pimentel, A. C., Pereira, A. T., Chu, V., Prazeres, D. M. F., Conde, J. P., IEEE Sensors Journal, 7(3), 415–416 (2007)Google Scholar