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Oblique-Incidence Reflectivity Difference and Fluorescence Imaging of Oligonucleotide and IgG Protein Microarrays

Published online by Cambridge University Press:  15 February 2011

James P. Landry ,
X. D. Zhu ,
X. W. Guo  and
J. P. Gregg
James P. Landry
Affiliation:
Department of Physics, University of California at Davis One Shields Avenue, Davis, CA 95616
X. D. Zhu
Affiliation:
Department of Physics, University of California at Davis One Shields Avenue, Davis, CA 95616
X. W. Guo
Affiliation:
Department of Physics, University of California at Davis One Shields Avenue, Davis, CA 95616
J. P. Gregg
Affiliation:
Department of Molecular Pathology, University of California at Davis, Sacramento, CA
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Abstract

We have constructed an oblique-incidence reflectivity difference (OI-RD) microscope for fluorescent label-free imaging of DNA and protein microarrays on standard glass substrates. Using both OI-RD and fluorescence images, we demonstrate a difference in wetting behavior of labeled and unlabeled IgG protein molecules deposited on an aldehyde-derivatized glass surface. The potential of fluorescent labeling agents to influence the properties of proteins highlights the need for label-free microarray detection techniques to supplement existing fluorescence methods. We also present OI-RD images of an oligonucleotide microarray after printing and washing procedures to demonstrate the use of OI-RD for non-destructive monitoring of changes in the optical properties of microarrays during processing.

Type
Research Article
Information
MRS Online Proceedings Library (OPL) , Volume 773: Symposium N – Biomicroelectromechanical Systems (BioMEMS) , 2003 , N7.5
Copyright
Copyright © Materials Research Society 2003

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