Hostname: page-component-cd9895bd7-gvvz8 Total loading time: 0 Render date: 2024-12-27T01:47:55.160Z Has data issue: false hasContentIssue false
  • English
  • Français

A Novel Pressure Indicator for Continuous Flow PCR Chip Using Micro Molded PDMS Pillar Arrays

Published online by Cambridge University Press:  01 February 2011

Yi Zhao  and
Xin Zhang
Yi Zhao
Affiliation:
Laboratory of Microsystems Technology, Department of Manufacturing Engineering, Boston University, 15 Saint Mary's Street, Boston, MA 02215, USA
Xin Zhang
Affiliation:
Laboratory of Microsystems Technology, Department of Manufacturing Engineering, Boston University, 15 Saint Mary's Street, Boston, MA 02215, USA
Get access

Abstract

DNA amplification is one of the most routine experiments carried out in biological laboratories. Continuous flow PCR chip releases biologists from their laborious exercises. The use of such chip is, however, hindered by costly expense of the syringe pump, which is used to maintain a constant flow rate. In this paper, we demonstrate a novel pressure indicator which makes up an in-line flow sensor in a continuous flow PCR chip. The pressure of PCR channel is achieved from pattern change in fabricated microstructures, and converted to volume flow rate. A polymeric PCR chip with such pressure indicators is presented. With a much less expense as compared to its conventional peers, this indicator has a wide potential for the use in the laboratories which runs daily activities like sequencing or mutagenesis.

Type
Research Article
Information
MRS Online Proceedings Library (OPL) , Volume 845: Symposium AA – Nanoscale Materials Science in Biology and Medicine , 2004 , AA5.10
Copyright
Copyright © Materials Research Society 2005

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

REFERENCE

[1] Kopp, M., de Mello, A., and Manz, A., Chemical amplification: Continuous-flow PCR on a chip. Science, 280(5366), 1046–1048, 1998.Google Scholar
[2] Oosterbroek, R. and et al., A micromachined pressure/flow-sensor. Sensors and Actuators A, 77(3), 167–177, 1999.Google Scholar
[3] Kurzweg, U. H., Jaeger, M. J., Flow rate determination in conduits using a thermal pulse technique. International Communications in Heat and Mass Transfer, 24(2), 211–221, 1997.Google Scholar
[4] Fuhr, G., Hagedorn, R., Muller, T., Benecke, W. and Wager, B., Pumping of water solutions in microfabricated electrohydrodynamic systems Proc. MEMS 92, Travemunde, Germany, 25–30, 1992.Google Scholar
[5] Yu, H., Li, B., Zhao, Y., and Zhang, X., Fabrication of three-dimensional SU-8 microstructures for MEMS applications, Proc. ASME 04, Anaheim, USA, in press, 2004.Google Scholar
[6] White, F. W., Fluid Mechanics, 3rd edn., McGraw-Hill, NewYork, NY, 1994.Google Scholar
[7] Pan, J.Y., Lin, P., Maseeh, F., and Senturia, S. D., Verification of FEM analysis of load-deflection methods for measuring mechanical properties of thin films, Tech. Dig. IEEE Solid-State Sensors and Actuator Workshop, Hilton Head, SC, 70–73, 1990.Google Scholar