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A Novel Injectable Chitosan Sponge Containing Brain Derived Neurotrophic Factor (BDNF) to Enhance Human Oligodendrocyte Progenitor Cells' (OPC) Differentiation

Published online by Cambridge University Press:  22 January 2014

Mina Mekhail ,
Qiao-Ling Cui ,
Guillermina Almazan ,
Jack Antel  and
Maryam Tabrizian
Mina Mekhail
Affiliation:
Biomedical Engineering, Faculty of Medicine, McGill University, Montreal, QC, Canada
Qiao-Ling Cui
Affiliation:
Neurology and Neurosurgery, Faculty of Medicine, McGill University, Montreal, QC
Guillermina Almazan
Affiliation:
Pharmacology and Therapeutics, Faculty of Medicine, McGill University, Montreal, QC
Jack Antel
Affiliation:
Neurology and Neurosurgery, Faculty of Medicine, McGill University, Montreal, QC
Maryam Tabrizian
Affiliation:
Biomedical Engineering, Faculty of Medicine, McGill University, Montreal, QC, Canada
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Abstract

We developed a rapidly-gelling chitosan sponge crosslinked with Guanosine 5'-Diphosphate (GDP). GDP has not been previously explored as an anionic crosslinker, and it was used in this application since the nucleoside guanosine has been shown to improve remyelination in situ, and thus its presence in the sponge composition was hypothesized to induce Oligodendrocyte Progenitor Cells' (OPC) differentiation. In addition to the chemical composition tailored to target OPCs, the developed chitosan sponge possesses a wide range of desirable physicochemical properties such as: rapid gelation, high porosity with interconnected pores, moduli of elasticity resembling that of soft tissue and cytocompatibility with many cell types. Moreover, protein encapsulation into the sponges was possible with high encapsulation efficiencies (e.g. BMP-7 and NT-3). In this study, BDNF was encapsulated in the chitosan sponges with an encapsulation efficiency greater than 80% and a sustained release over a 16-day period was achieved. We demonstrate here for the first time, the attachment of human fetal OPCs to the sponges and their differentiation after 12 days of culture. Overall, this newly-introduced injectable sponge is a promising therapeutic modality that can be used to enhance remyelination post-spinal cord injuries.

Keywords

biomaterialpolymerrapid solidification
Type
Articles
Information
MRS Online Proceedings Library (OPL) , Volume 1621: Symposium H/C/D/J – Advances in Structures, Properties and Applications of Biological and Bioinspired Materials , 2014 , pp. 127 - 132
Copyright
Copyright © Materials Research Society 2014 

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References

REFERENCES

Mekhail, M., Almazan, G., Tabrizian, M., Progress in neurobiology, 96 (2012) 322–339.CrossRefGoogle Scholar
Franklin, R.J., Ffrench-Constant, C., Nat Rev Neurosci, 9 (2008) 839–855.CrossRefGoogle Scholar
Mekhail, M., Daoud, J., Almazan, G., Tabrizian, M., Adv Healthc Mater, (2013).Google Scholar
Jagielska, A., Norman, A.L., Whyte, G., Vliet, K.J., Guck, J., Franklin, R.J., Stem Cells Dev, 21 (2012) 2905–2914.CrossRefGoogle Scholar
Ichihara, K., Taguchi, T., Shimada, Y., Sakuramoto, I., Kawano, S., Kawai, S., J Neurotrauma, 18 (2001) 361–367.CrossRefGoogle Scholar
Oakland, R., Hall, R., Wilcox, R., Barton, D., Proceedings of the Institution of Mechanical Engineers, Part H: Journal of Engineering in Medicine, 220 (2006) 489–492.CrossRefGoogle Scholar
Cui, Q.L., D'Abate, L., Fang, J., Leong, S.Y., Ludwin, S., Kennedy, T.E., Antel, J., Almazan, G., Stem Cells Dev, 21 (2012) 1831–1837.CrossRefGoogle Scholar
Jiang, S., Khan, M.I., Lu, Y., Wang, J., Buttigieg, J., Werstiuk, E.S., Ciccarelli, R., Caciagli, F., Rathbone, M.P., Neuroreport, 14 (2003) 2463–2467.CrossRefGoogle Scholar
McTigue, D.M., Horner, P.J., Stokes, B.T., Gage, F.H., J Neurosci, 18 (1998) 5354–5365.CrossRefGoogle Scholar