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Novel Fluorescent Labels Prepared by Layer-by-Layer Assembly on Colloids for Biodetection Systems

Published online by Cambridge University Press:  21 March 2011

Wenjun Yang ,
Dieter Trau ,
Reinhard Renneberg ,
Nai-Teng Yu  and
Frank Caruso
Wenjun Yang
Affiliation:
Department of Chemistry, The Hong Kong University of Science and Technology, Clear Water Bay, Kowloon, SAR Hong Kong, People's Republic of China
Dieter Trau
Affiliation:
Department of Chemistry, The Hong Kong University of Science and Technology, Clear Water Bay, Kowloon, SAR Hong Kong, People's Republic of China
Reinhard Renneberg
Affiliation:
Department of Chemistry, The Hong Kong University of Science and Technology, Clear Water Bay, Kowloon, SAR Hong Kong, People's Republic of China
Nai-Teng Yu
Affiliation:
Department of Chemistry, The Hong Kong University of Science and Technology, Clear Water Bay, Kowloon, SAR Hong Kong, People's Republic of China
Frank Caruso
Affiliation:
Max Planck Institute of Colloids and Interfaces, D-14424 Potsdam, Germany
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Abstract

Fluorescent polystyrene microparticles with different sizes were produced by the consecutive assembly of fluorescently labeled polyelectrolytes using the layer-by-layer self-assembly method. Film growth was characterized by microelectrophoresis and fluorescence microscopic image (FMI) analysis. Alternating negative and positive ζ-potentials with deposition of each successive polyelectrolyte layer demonstrated that the alternate adsorption of polyelectrolytes was achieved. FMI analysis provided direct measurement of the fluorescence intensity of single microparticles. The subsequent deposition of a protein (immunoglobulin G, IgG) layer onto the fluorescent microparticles was confirmed by a sandwich immunoassay.

Type
Research Article
Information
MRS Online Proceedings Library (OPL) , Volume 667: Symposium G – Luminescence and Luminescent Materials , 2001 , G5.5
Copyright
Copyright © Materials Research Society 2001

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References

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