Hostname: page-component-586b7cd67f-t8hqh Total loading time: 0 Render date: 2024-11-23T12:31:14.664Z Has data issue: false hasContentIssue false
  • English
  • Français

Novel and Unique Matrix Design for Osteochondral Tissue Engineering

Published online by Cambridge University Press:  12 March 2014

Deborah L. Dorcemus  and
Syam P. Nukavarapu
Deborah L. Dorcemus
Affiliation:
Institute for Regenerative Engineering, University of Connecticut Health Center Farmington, CT 06030, U.S.A Biomedical Eng., University of Connecticut Storrs, CT 06269, U.S.A
Syam P. Nukavarapu
Affiliation:
Institute for Regenerative Engineering, University of Connecticut Health Center Farmington, CT 06030, U.S.A Biomedical Eng., University of Connecticut Storrs, CT 06269, U.S.A Materials Science & Eng. University of Connecticut Storrs, CT 06269, U.S.A Department of Orthopedic Surgery University of Connecticut Health Center Farmington, CT 06030, U.S.A
Get access

Abstract

Osteochondral (OC) tissue is comprised of articular cartilage, the subchondral bone and the central cartilage-bone interface. To facilitate proper regeneration, an equally complex and multiphasic matrix must be used. Although mono-phasic and bi-phasic matrices were previously applied, they failed to establish the OC interface upon regeneration. In this study, we designed and developed a novel matrix with increasing pore volume from one end to other, along the matrix length. For this matrix polylactide-co-glycolide (PLGA) 85:15 microspheres were combined with a water-soluble porogen in a layer-by-layer fashion and thermally sintered. The resulting matrix was then porogen-leached to form a gradiently-porous structured matrix. The formation of this gradient pore structure was established using Micro-Computed Tomography (μCT) scanning. A biodegradable hydrogel was infiltrated into the structure to form a unique OC matrix where the microsphere and hydrogel phases co-exist with opposing gradients. When the individual phases are associated with osteogenic and chondrogenic growth factors, the structureinduced factor delivery might provide the spatially controlled factor delivery necessary to regenerate osteochondral tissue structure. Overall, we designed a gradient matrix system that is expected to support osteochondral tissue engineering while forming a seamless interface between the cartilage and the bone matrix.

Keywords

tissuebonebiomimetic (assembly)
Type
Articles
Information
MRS Online Proceedings Library (OPL) , Volume 1621: Symposium H/C/D/J – Advances in Structures, Properties and Applications of Biological and Bioinspired Materials , 2014 , pp. 17 - 23
Copyright
Copyright © Materials Research Society 2014 

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

REFERENCES

Nukavarapu, SP, Dorcemus, DL (2013) Osteochondral tissue engineering: Current strategies and challenges. Biotechnology Advances 31: 706–721.CrossRefGoogle ScholarPubMed
Shao, X, Goh, J, Hutmacher, DW, Lee, EH, Zigang, GE (2006) Repair of Large Articular Osteochondral Defects Using Hybrid Scaffolds and Bone Marrow-Derived Mesenchymal Stem Cells in a Rabbit Model. Tissue Eng 12.CrossRefGoogle Scholar
Liu, M, Yu, X, Huang, F, Cen, S, Zhong, G, et al. . (2013) Tissue Engineering Stratified Scaffolds for Articular Cartilage and Subchondral Bone Defects Repair. Orthopedics 36: 868–873.CrossRefGoogle ScholarPubMed
Mohan, N, Dormer, NH, Caldwell, KL, Key, VH, Berkland, CJ, Detamore, MS (2011) Continuous gradients of material composition and growth factors for effective regeneration of the osteochondral interface. Tissue Eng Part A 17.CrossRefGoogle ScholarPubMed
Chu, CR, Coutts, RD, Yoshioka, M, Harwood, FL, Monosov, AZ, et al. . (1995) Articular-Cartilage Repair Using Allogeneic Perichondrocyte-Seeded Biodegradable Porous Polylactic Acid (PLA): A Tissue Engineering Study. Journal of Biomedical Materials Research 29.CrossRefGoogle ScholarPubMed
Gao, JZ, Dennis, JE, Solchaga, LA, Awadallah, AS, Goldberg, VM, et al. . (2001) Tissueengineered fabrication of an osteochondral composite graft using rat bone marrowderived mesenchymal stem cells. Tissue Engineering 7.CrossRefGoogle Scholar
Malda, J, Woodfield, TBF, van der Vloodt, F, Wilson, C, Martens, DE, et al. . (2005) The effect of PEGT/PBT scaffold architecture on the composition of tissue engineered cartilage. Biomaterials 26.CrossRefGoogle ScholarPubMed
Getgood, AMJ, Kew, SJ, Brooks, R, Aberman, H, Simon, T, et al. . (2012) Evaluation of earlystage osteochondral defect repair using a biphasic scaffold based on a collagenglycosaminoglycan biopolymer in a caprine model. Knee 19.CrossRefGoogle Scholar
Schek, RM, Taboas, JM, Segvich, SJ, Hollister, SJ, Krebsbach, PH (2004) Engineered osteochondral grafts using biphasic composite solid free-form fabricated scaffolds. Tissue Engineering 10.CrossRefGoogle ScholarPubMed
Keeney, M, Pandit, A (2009) The Osteochondral Junction and Its Repair via Bi-Phasic Tissue Engineering Scaffolds. Tissue Engineering Part B-Reviews 15.CrossRefGoogle ScholarPubMed
Athanasiou, KA, Darling, EM, Hu, JC (2009) Articular Cartilage Tissue Engineering; Athanasiou, K, editor: Morgan & Claypool. 168 p.Google Scholar
Marieb, EN (2001) Human Anatomy & Physiology; Ueno, K, editor. San Francisco: Benjamin Cummings.Google Scholar
Amini, AR, Adams, DJ, Laurencin, CT, Nukavarapu, SP (2012) Optimally porous and biomechanically compatible scaffolds for large-area bone regeneration. Tissue Eng Part A 18.CrossRefGoogle ScholarPubMed
Igwe, J, Mikael, PE, Nukavarapu, SP (2014) Design, fabrication and in vitro evaluation of novel polymer-hydrogel hybrid scaffold for bone tissue engineering. J Regen Med Tissue Engin. 2.Google Scholar