Hostname: page-component-cd9895bd7-7cvxr Total loading time: 0 Render date: 2024-12-27T02:29:26.940Z Has data issue: false hasContentIssue false
  • English
  • Français

Nonthermal Bioplasma Diagnostics and its Applications to the Microbial and Living Cells

Published online by Cambridge University Press:  21 May 2012

Eun-Ha Choi ,
Gyungsoon Park ,
Ku Y. Baik ,
Ran J. Jung ,
Nagendra K. Kaushik ,
Guangsup Cho ,
Geon J. Lee ,
Bong J. Park ,
Byoungchoo Park  and
Gi C. Kwon
...Show all authors
Eun-Ha Choi*
Affiliation:
Department of Electrophysics & Plasma Bioscience, Plasma Bioscience Research Center, Kwangwoon University, Seoul 139-701, Korea
Gyungsoon Park
Affiliation:
Department of Electrophysics & Plasma Bioscience, Plasma Bioscience Research Center, Kwangwoon University, Seoul 139-701, Korea
Ku Y. Baik
Affiliation:
Department of Electrophysics & Plasma Bioscience, Plasma Bioscience Research Center, Kwangwoon University, Seoul 139-701, Korea
Ran J. Jung
Affiliation:
Department of Electrophysics & Plasma Bioscience, Plasma Bioscience Research Center, Kwangwoon University, Seoul 139-701, Korea
Nagendra K. Kaushik
Affiliation:
Department of Electrophysics & Plasma Bioscience, Plasma Bioscience Research Center, Kwangwoon University, Seoul 139-701, Korea
Guangsup Cho
Affiliation:
Department of Electrophysics & Plasma Bioscience, Plasma Bioscience Research Center, Kwangwoon University, Seoul 139-701, Korea
Geon J. Lee
Affiliation:
Department of Electrophysics & Plasma Bioscience, Plasma Bioscience Research Center, Kwangwoon University, Seoul 139-701, Korea
Bong J. Park
Affiliation:
Department of Electrophysics & Plasma Bioscience, Plasma Bioscience Research Center, Kwangwoon University, Seoul 139-701, Korea
Byoungchoo Park
Affiliation:
Department of Electrophysics & Plasma Bioscience, Plasma Bioscience Research Center, Kwangwoon University, Seoul 139-701, Korea
Gi C. Kwon
Affiliation:
Department of Electrophysics & Plasma Bioscience, Plasma Bioscience Research Center, Kwangwoon University, Seoul 139-701, Korea
Han S. Uhm
Affiliation:
Department of Electrophysics & Plasma Bioscience, Plasma Bioscience Research Center, Kwangwoon University, Seoul 139-701, Korea
*
*[email protected]
Get access

Abstract

We have investigated the nonthermal bioplasma sources and their characteristics as well as their interactions with biological cells. The electron temperature and plasma density are measured to be about 1.5 eV and 3×1012 cm-3 , respectively, for the direct palsma jet under Ar gas flow. The hydroxyl radical density has also been investigated and measured to be maximum value of about 3 ×1015 cm-3 and 8 ×1014 cm-3 in the direct plasma jet and dielectric barrier discharge bioplasma, respectively, by the ultraviolet optical absorption spectroscopy. Herein, we have investigated the basic interactions of these nonthermal bioplasma with the living organisms in morphological and biomolecular aspects. We found that the secondary electron emision coefficient of the biological surface has been drastically increased by atmospheric bioplasma, which indicates the biological surface to be oxidized especially by the hydroxyl (OH) radical species. In order to elucidate the basic mechanisms for the cell shrinking and apoptosis leading to a cell death by the nonthermal bioplasma, the cell membrane potential has been estimated based on the ROS density as well as cell capacitances. It is also found that the molecular electron energy band structure in the biological cells have been shifted closer toward the vacuum surface and accordingly their central energy of molecular band becomes small by the nonthermal bioplasma due to cell oxidation caused by OH radicals.

Keywords

biomaterialAuger electron spectroscopy (AES)biological
Type
Research Article
Information
MRS Online Proceedings Library (OPL) , Volume 1469: Symposium WW – Plasma Processing and Diagnostics for Life Sciences. , 2012 , mrss12-1469-ww01-02
Copyright
Copyright © Materials Research Society 2012

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

REFERENCES

1. Hong, Y. C., Uhm, H. S., and Yi, W. J., Appl. Phys. Lett. 93, 051504 (2008).10.1063/1.2969287Google Scholar
2. Bandyopadhyay, Uday, Das, Dipak and Banerjee, Ranajit K., “ Reactive Oxygen Species: Oxidative Damage andPathogenesis ”, Current Science 77, P 658 (1999).Google Scholar
3. Hong, Y. J., Nam, C. J., Song, K. B., Cho, G., Uhm, H. S., Choi, D. I. and Choi, E. H., JINST 7, C03046(2012).Google Scholar
4. Park, G., Ryu, Y. H., Hong, Y. J., Choi, E. H., and Uhm, H.S., Appl. Phys. Lett. 100, 063703 (2012).10.1063/1.3684632Google Scholar
5. Nagendra, Kaushik, K., Uhm, H. S., and Choi, E. H., Appl. Phys. Lett. 100, 084102 (2012).Google Scholar
6. Choi, E. H., Oh, H. J., Kim, Y. G., Ko, J. J., Lim, J. Y., Kim, J. G., Kim, D. I., Cho, G., and Kang, S. O., Jpn. J. Appl. Phys. 37, 7015 (1998).10.1143/JJAP.37.7015Google Scholar
7. Uhm, H. S., Choi, E. H., and Cho, G., Appl. Phys. Lett. 94, 0031501 (2009).10.1063/1.3073983Google Scholar
8. Uhm, H. S., Hong, Y. J., Choi, E. H., and Kim, D. Y., Appl. Phys. Lett. 98, 061501 (2011).10.1063/1.3554433Google Scholar
9. Kwon, B. G. and Yoon, J., J. Korean Ind. Eng. Chem, 20, 593(2009).Google Scholar
10. Dieke, G. H. and Crosswhite, H. M., “The ultraviolet bands of OH Fundamental data ”, J.Quant. Spectrosc. Radiat. Transfer, 2, P 97 (1961).10.1016/0022-4073(62)90061-4Google Scholar
11. Benstaali, B., Boubert, P., Cheron, B. G., Addou, A., and Brisset, J. L., Plasma Chemistry and Plasma Processing 22, 553 (2002).10.1023/A:1021371529955Google Scholar
12. Ahn, J. C., Kim, S. B., Cho, T. S., Choi, M. C., Joh, D. G., Moon, M. W., Seo, Y., Kang, S. O., Cho, G. S., Cho, E. H., and Uhm, H. S., Jpn. J. Appl.Phys., 41, 860 (2002).10.1143/JJAP.41.860Google Scholar
13. Choi, E. H., Cho, T. S., Cho, D. S., Choi, M. C., et al. . Jpn. J. Appl. Phys. 38, 6073 (1999).10.1143/JJAP.38.6073Google Scholar
14. Engler, A. J., Rehfeldt, F., Sen, S., and Discher, D. E., “Microtissue Elasticity: Measurements by Atomic Force Microscopy and Its Influence on Cell Differentiation”, Chapter 22, METHODS IN CELL BIOLOGY , VOL. 83, p. 521 (Elsevier, 2007).Google Scholar