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Nanotextured Electrical Immunoassays for Ultrasensitive Protein Detection

Published online by Cambridge University Press:  20 July 2011

Timothy O. Mertz ,
Krishna Vattipalli ,
Tom Barrett ,
John Carruthers  and
Shalini Prasad
Timothy O. Mertz
Affiliation:
Department of Electrical Engineering and Computer Science, Wichita State University, Wichita, Kansas 67260, U.S.A.
Krishna Vattipalli
Affiliation:
Department of Electrical Engineering and Computer Science, Wichita State University, Wichita, Kansas 67260, U.S.A.
Tom Barrett
Affiliation:
Department of Medicine, Oregon Health and Sciences University, Portland, Oregon 97239, U.S.A.
John Carruthers
Affiliation:
Department of Physics, Portland State University, Portland, Oregon 97201, U.S.A.
Shalini Prasad
Affiliation:
Department of Electrical Engineering and Computer Science, Wichita State University, Wichita, Kansas 67260, U.S.A.
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Abstract

This paper describes the development of nanomonitors, which are electrical immunoassays for detection of multiple protein biomarkers. These devices are hybrid sensors with micro-fabricated electrode arrays on a silicon substrate, and integrated nanoporous alumina membranes to provide protein confinement and signal amplification. The disease biomarkers C-reactive protein and Myeloperoxidase have been detected by the nanomonitors in ultra-low concentrations. Proteins were detected in pure samples, human serum, and patient blood samples. The detection accuracy and sensitivity of the nanomonitors in patient samples was comparable to the Enzyme Linked Immunosorbent Assay (ELISA) method of protein detection. Nanomonitors provide the additional benefits of being rapid, label-free, sensitive, and cost effective, providing improvements over traditional protein detection methods, and having potential applications in disease diagnosis.

Keywords

nanoscalebiomedicalsensor
Type
Research Article
Information
MRS Online Proceedings Library (OPL) , Volume 1346: Symposium AA – Micro- and Nanofluidic Systems for Materials Synthesis, Device Assembly and Bioanalysis , 2011 , mrss11-1346-aa02-06
Copyright
Copyright © Materials Research Society 2011

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References

REFERENCES

1 Nagaraj, V, Aithal, S, Eaton, S, Bothara, M, Wiktor, P, and Prasad, S. NanoMonitor: a miniature electronic biosensor for glycan biomarker detection. Nanomedicine, vol. 5, no. 3, pp. 369–378, 2010.Google Scholar
2 Bothra, M, Venkatraman, V, Reddy, R, Barrett, T, Carruthers, J, and Prasad, S. Nanomonitors: electrical immunoassays for protein biomarker profiling. Nanomedicine, vol. 3, pp. 423–426, 2008.Google Scholar
3 Reddy, R, Bothra, M, Barrett, T, Carruthers, J, and Prasad, S. Nanomonitors: protein biosensors for rapid analyte analysis. IEEE Sensors, vol. 8, pp. 720–723, 2008.Google Scholar
4 Vulnerable Plaques: A Brief Review of the Concept and Proposed Approaches to Diagnosis and Treatment http://www.ahrq.gov/Clinic/ta/placque/placque.pdf, 2004.Google Scholar
5 American Heart Association, "Inflammation, Heart Disease and Stroke: The Role of C-Reactive Protein", retrieved from: http://www.americanheart.org/presenter.jhtml?identifier=4648, 2003.Google Scholar
6 Chebotareva, NA, Kurganov, BI, Livanova, NB. Biochemical Effects of Molecular Crowding. Biochemistry 2004; 69(11):1239–51.Google Scholar