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Nanostructured biofunctionalized polyurethanes for applications in regenerative medicine

Published online by Cambridge University Press:  19 April 2012

Sebastian Kruss  and
Tobias Wolfram
Sebastian Kruss
Affiliation:
Department of New Materials and Biosystems, Max Planck Institute for Intelligent Systems, Heisenbergstr. 3, 70569 Stuttgart, Germany Biophysical Chemistry, Institute of Physical Chemistry, University of Heidelberg, Im Neuenheimer Feld 253, 69120 Heidelberg, Germany
Tobias Wolfram
Affiliation:
Hochschule Furtwangen, Jakob-Kienzle-Str. 17, 78054 Villingen-Schwenningen, Germany
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Abstract

Polyurethane (PU) materials are used in a wide variety of implantable devices and technologies, e.g. stents, breast augmentation, nose surgery and bladder reconstruction. Despite the excellent chemical control for manufacturing bulk materials and the good biocompatibility, a major challenge remains interfacing of PU with biological environments. A chemically controlled surface engineering approach could improve desired protein adsorption processes and cellular interactions within different tissues, preventing uncontrolled events of the implant especially in early stages shortly after surgical procedures.

To gain better control over the PU surfaces we polymerized different bulk PU materials and developed a transfer-nanolithography technique to deposit inorganic Au-nanoparticles with defined structural features on the PU surface. Different nanoparticle patterns were transferred and analyzed by scanning electron microscopy (SEM) and high-resolution transmission electron microscopy (HR-TEM). Topographical features of PU substrates were investigated by atomic force microscopy (AFM). Transferred Au-nanoparticles showed high stability on PU substrates even under extreme sonication conditions. In a final step, those nanoparticles were functionalized with peptides to facilitate cellular adhesion under physiologically relevant conditions. As proof of concept, rat embryonic fibroblast cells were cultured on a peptide functionalized PU interface and investigated by SEM.

In conclusion, we developed a versatile method to prepare nanostructured and biofunctionalized PUs. These PUs showed good stability characteristics and in vitro biocompatibility in cell culture assays.

Keywords

biomedicalnanoscalepolymer
Type
Research Article
Information
MRS Online Proceedings Library (OPL) , Volume 1417: Symposium KK – Biomaterials for Tissue Regeneration , 2012 , mrsf11-1417-kk03-36
Copyright
Copyright © Materials Research Society 2012

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References

REFERENCES

1) Burke, A. and Hasirci, N., “Polyurethanes in biomedical applications,” Adv Exp Med Biol 2004, 553, 83–101.Google Scholar
2) Zdrahala, I.J. and Zdrahala, R.J., “Biomedical applications of polyurethanes: a review of past promises, present realities, and a vibrant future.” J Biomater Appl 1999, 14(1), 67–90.Google Scholar
3) Ratner, B. D. and Bryant, S. J., “Biomaterials: where we have been and where we are going,” Annu Rev Biomed Eng 2004, 6, 41–75.Google Scholar
4) Kruss, S., Wolfram, T., Martin, R., Neubauer, S., Kessler, H., and Spatz, J.P., “Stimulation of cell adhesion at nanostructured teflon interfaces,” Adv Mat 2010, 22(48), 5499–5506.Google Scholar
5) Spatz, J., Mossmer, S., Hartmann, C., and Möller, M., “Ordered deposition of inorganic clusters from micellar block copolymer films,” Langmuir 2000, 16(2), 407–415.Google Scholar
6) Hsu, S. H., Tang, C., and Tseng, H., “Gold nanoparticles induce surface morphological transformation in polyurethane and affect the cellular response,” Biomacromolecules 2008, 9, 241–248.Google Scholar