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Nanostructure analysis of protein thin film prepared by wet and dry processes

Published online by Cambridge University Press:  01 February 2011

I. Taketani ,
S. Nakayama ,
S. Nagare  and
M. Senna
I. Taketani
Affiliation:
Keio University, Faculty of Science and Technology, 3–14–1 Hiyoshi, Kohoku-ku, Yokohama 223–8522, Japan TEL: 045–566–1589, FAX: 045–564–0950, e-mail: [email protected]
S. Nakayama
Affiliation:
Keio University, Faculty of Science and Technology, 3–14–1 Hiyoshi, Kohoku-ku, Yokohama 223–8522, Japan TEL: 045–566–1589, FAX: 045–564–0950, e-mail: [email protected]
S. Nagare
Affiliation:
Nara Machinery Co., Ltd., 2–5–7 Jonan-jima, Ohta-ku, Tokyo, 143‐0002, Japan
M. Senna
Affiliation:
Keio University, Faculty of Science and Technology, 3–14–1 Hiyoshi, Kohoku-ku, Yokohama 223–8522, Japan TEL: 045–566–1589, FAX: 045–564–0950, e-mail: [email protected]
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Abstract

Silk fibroin (SF) and bovine serum albumin (BSA) thin films were deposited on Si(100) substrate either by colloid casting method or by pulsed laser deposition (PLD). Emphasis is laid on the comparison of nanostructures, which developed during the deposition. AFM diagrams were evaluated to quantify the nanostructures, parallel and perpendicular to the substrate, by the average wavelength of the profilogram (Wa ) and surface roughness (Rrms ), respectively. The film thickness increased with increasing the concentration of colloids or laser deposition time. The value of Wa increased with the film thickness. We note, however, that the value Wa becomes much less sensitive with decreasing the scanning area due to decreasing probability of the abnormally large agglomerates of the nanometric units. Probability of the agglomeration growth as a consequence of the surface adhesion is higher with higher concentration of random coil, followed by β-sheet and α-helix. BSA with smaller primary units and lower probability of agglomeration give more uniform films.

Type
Research Article
Information
MRS Online Proceedings Library (OPL) , Volume 849: Symposium KK – Kinetics-Driven Nanopatterning on Surfaces , 2004 , KK3.10
Copyright
Copyright © Materials Research Society 2005

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References

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