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Nano-scale Debye Capacitive Sensors for Highly Sensitive, Label-free, Nucleic Acid Analysis

Published online by Cambridge University Press:  01 February 2011

Manu Sebastian Mannoor ,
Teena James ,
Dentcho V. Ivanov ,
Bill Braunlin  and
Les Beadling
Manu Sebastian Mannoor
Affiliation:
[email protected]@gmail.com, NJIT, Microelectronics Research Center, Newark, New Jersey, United States
Teena James
Affiliation:
[email protected], NJIT, Microelectronics Research Center, Newark, New Jersey, United States
Dentcho V. Ivanov
Affiliation:
[email protected], NJIT, Microelectronics Research Center, Newark, New Jersey, United States
Bill Braunlin
Affiliation:
[email protected], Rational Affinity Devices LLC, Newark, New Jersey, United States
Les Beadling
Affiliation:
[email protected], Rational Affinity Devices LLC, United States
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Abstract

An electrochemical capacitive sensor with electrode separation in the order of the Electrical Double Layer width (Debye length) of the analyte solution is presented for extremely sensitive and label-free analysis of Nucleic Acids. As the Electrical Double Layers (EDL) from both the capacitive electrodes interact and overlap each other in the reduced space confinement, the contribution from the electrode polarization effects and noises due to bulk sample resistance are found to be minimized. The dielectric property changes during hybridization reaction were measured using 10-mer nucleotide sequences. A 30-45% change in relative permittivity (capacitance) was observed due to DNA hybridization at 10Hz.

Keywords

nanoscalemicroelectro-mechanical (MEMS)biomedical
Type
Research Article
Information
MRS Online Proceedings Library (OPL) , Volume 1138: Symposium FF – Nanofunctional Materials, Structures and Devices for Biomedical Applications , 2008 , 1138-FF06-01
Copyright
Copyright © Materials Research Society 2009

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References

REFERENCES

[1] Lo?hndorf, M., Schlecht, U., Gronewold, T.M.A., Malave, A., and Tewes, M., “Microfabricated high-performance microwave impedance biosensors for detection of aptamer-protein interactions,” Applied Physics Letters, vol. 87, no. 24, pp. 1–3, 2005.Google Scholar
[2] Sanabria, H. and Miller, J.H., “Relaxation processes due to the electrode-electrolyte interface in ionic solutions,” Physical Review E - Statistical, Nonlinear, and Soft Matter Physics, vol. 74, no. 5, 2006.Google Scholar
[3] Oleinikova, A., Sasisanker, P., and Weinga?rtner, H., “What can really be learned from dielectric spectroscopy of protein solutions? A case study of ribonuclease A,” Journal of Physical Chemistry B, vol. 108, no. 24, pp. 8467–8474, 2004.Google Scholar
[4] Hunter, R.J. Introduction to Modern Colloid Science, 1993.Google Scholar
[5] Nuzzo, R.G. and Allara, D.L., “Adsorption of bifunctional organic disulfides on gold surfaces,” Journal of the American Chemical Society, vol. 105, no. 13, pp. 4481–4483, 1983.Google Scholar
[6] Wink, T., Van Zuilen, S.J., Bult, A., and Van Bennekom, W.P., “Self-assembled monolayers for biosensors,” Analyst, vol. 122, no. 4, pp. 43R–50R, 1997.Google Scholar
[7] Collard, D.M. and Fox, M.A., “Use of electroactive thiols to study the formation and exchange of alkanethiol monolayers on gold,” Langmuir, vol. 7, no. 6, pp. 1192–1197, 1991.Google Scholar
[8] Liu, Y.F., Yang, Y.C., and Lee, Y.L., “Assembly behavior and monolayer characteristics of OH-terminated alkanethiol on Au(111): In situ scanning tunneling microscopy and electrochemical studies,” Nanotechnology, vol. 19, no. 6, 2008.Google Scholar