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Nanoparticles Amplified QCM Sensor for Enzyme Activity Evaluation

Published online by Cambridge University Press:  13 February 2015

M. Stoytcheva ,
R. Zlatev ,
G. Montero ,
B. Valdez  and
M. Schorr
M. Stoytcheva
Affiliation:
Engineering Institute of the Autonomous University of Baja California, Mexicali, Mexico
R. Zlatev
Affiliation:
Engineering Institute of the Autonomous University of Baja California, Mexicali, Mexico
G. Montero
Affiliation:
Engineering Institute of the Autonomous University of Baja California, Mexicali, Mexico
B. Valdez
Affiliation:
Engineering Institute of the Autonomous University of Baja California, Mexicali, Mexico
M. Schorr*
Affiliation:
Engineering Institute of the Autonomous University of Baja California, Mexicali, Mexico
*
*Presenting author’s email: [email protected]
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Abstract

This investigation introduces a new very simple and efficient approach for QCM sensor response amplification, developed for hydrolases activity determination. For this purpose, the QCM crystal surface was modified with nanoparticles loaded enzyme substrate. During the enzymatic substrate degradation, the heavier nanoparticles were also released from the sensitive layer together with the substrate degradation products. Nanoparticles removal resulted in QCM signal amplification due to the higher nanoparticles specific mass compared with the specific mass of the substrate.

The suggested concept was successfully applied for creating of simple biosensing platforms for trypsin and lipase activity determination in real time using respectively SiO2 nanoparticles loaded olive oil and Ag nanoparticles loaded gelatin as enzyme substrates. Up to 10 times amplification of the QCM signal was reached applying the proposed approach compared with the common one.

Keywords

piezoelectricsensornanostructure
Type
Articles
Information
MRS Online Proceedings Library (OPL) , Volume 1763: Symposium 2B – Materials for Biosensor Applications , 2015 , IMRC2014-S2B-P001
Copyright
Copyright © Materials Research Society 2015 

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