Hostname: page-component-cd9895bd7-q99xh Total loading time: 0 Render date: 2024-12-27T01:38:51.321Z Has data issue: false hasContentIssue false
  • English
  • Français

Nanomonitors: Electrical Immunoassays for Protein Biomarker Profiling

Published online by Cambridge University Press:  01 February 2011

Manish Bothara ,
Ravi K Reddy ,
Thomas Barrett ,
John Carruthers  and
Shalini Prasad
Manish Bothara
Affiliation:
[email protected], Portland State University, Electrical and Computer Engineering, 1900 SW Fourth Avenue Bldg,, Wilsonville, OR, 97201, United States
Ravi K Reddy
Affiliation:
[email protected], Portland State University, Electrical and Computer Engineering, Portland, OR, 97201, United States
Thomas Barrett
Affiliation:
[email protected], Oregon Health and Science University, Veteran Affairs, Portland, OR, 97201, United States
John Carruthers
Affiliation:
[email protected], Portland State University, Physics Department, Portland, OR, 97201, United States
Shalini Prasad
Affiliation:
[email protected], Portland State University, Electrical and Computer Engineering, Portland, OR, 97201, United States
Get access

Abstract

The objective of this research is to develop a “point-of-care” device for early disease diagnosis through protein biomarker characterization. Here we present label-free, high sensitivity detection of proteins with the use of electrical immunoassays that we call Nanomonitors. The basis of the detection principle lies in the formation of an electrical double layer and its perturbations caused by proteins trapped in a nanoporous alumina membrane over a microelectrode array platform. High sensitivity and rapid detection of two inflammatory biomarkers, C-reactive protein (CRP) and Myeloperoxidase (MPO) in pure and clinical samples through label-free electrical detection were achieved. The performance metrics achieved by this device makes it suitable as a “lab-on-a-chip” device for protein biomarker profiling and hence early disease diagnosis.

Keywords

biomedicallithography (removal)nanostructure
Type
Research Article
Information
MRS Online Proceedings Library (OPL) , Volume 1106: Symposium PP – The Business of Nanotechnology , 2008 , 1106-PP03-02
Copyright
Copyright © Materials Research Society 2008

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

1 Darain, F., Park, D. S., Park, J. S. and Shim, Y. B., Biosensors & Bioelectronics, Vol. 19, pp.1245–52, 2004.Google Scholar
2 Hahm, J., and Lieber, C. M., Nano Letters, Vol. 4, No. 1, pp. 51–4, 2004.10.1021/nl034853bGoogle Scholar
3 Nam., J. M., Thaxton, C. S., and Mirkin, C. A., Science, Vol. 301, No. 5641, pp.1884–86, 2004.Google Scholar
4 Niwa, O., Morita, M., and Takei, H., Anal.Chem., Vol. 62, pp. 447–56, 1990.Google Scholar
5 Abeloff, M. D., Armitage, J. O., Lichter, A. S. and Niederbuber, J. E., Clinical Oncology, Churchill Livingstone, New York, 2000.Google Scholar
6 Chou, S. F., Hsu, W. L., Hwang, J. M. and Chen, C. Y., Biosens. Bioelectron., Vol. 19, pp.999–1005, 2004.Google Scholar
7 Arenkov, P., et al., Anal. Biochem., vol. 278, pp.123–31, 2000.10.1006/abio.1999.4363Google Scholar
8 Prasad, S., Zhang, X., Ozkan, C. S., Ozkan, M., Neuron-based microarray sensors for environmental sensing. Electrophoresis. 2004. 25(21-22): p.3746–60.Google Scholar
9 Prasad, S., and Quiano, J., Biosens. Bioelectron. 2004. 21(7): p.1219–29.Google Scholar
10 Cui, Y., Wei, Q. Q., Park, H. K., and Lieber, C. M., Nanowire nanosensors for highly sensitive and selective detection of biological and chemical species. Science. 2001. 293: p.1289–92.Google Scholar
11 Campagnolo, C., et al., Real-Time, label-free monitoring of tumor antigen and serum antibody interactions. J. Biochem. Biophys. Methods, 2004. 61: p.283–98.Google Scholar
12 Etzioni, R., et al., The case for early detection. Nat. Rev. Cancer. 2003. 3: p.243–52.Google Scholar
13 Zheng, G., Patolsky, F., Cui, Y., Wang, W. U., and Lieber, C. M., Nature. 2002. 23(10): p.1294–01.Google Scholar
14 Li, P., Muller, F., and Gosele, U., Electrochem. Solid-State Lett, 3:131, 2000.Google Scholar
15 MacDonald, J. R., Journal of Chemical Physics, 22:1317–1322, 1954.Google Scholar
16 Ridker, P. M., Am Heart J, 148(1 Suppl):S19–26, 2004.Google Scholar
17 Shah, S. H., and Newby, L. K., Cardiol Rev, 11(4):169–179, 2003.10.1097/01.CRD.0000077906.74217.6EGoogle Scholar