Hostname: page-component-78c5997874-8bhkd Total loading time: 0 Render date: 2024-11-05T04:21:11.433Z Has data issue: false hasContentIssue false
  • English
  • Français

Nano Monitors for Identification of Vulnerable Cardio-vascular Plaque

Published online by Cambridge University Press:  01 February 2011

Shalini Prasad ,
Thomas Barrett  and
John Carruthers
Shalini Prasad
Affiliation:
[email protected], Portland State University, ECE, 160-11, FAB 1900 SW 4th ave, Portland, OR, 97201, United States, 503-725-3223
Thomas Barrett
Affiliation:
[email protected], Oregon Health Sciences University, Portland, OR, 97209, United States
John Carruthers
Affiliation:
[email protected], Portland State University, Physics, Portland, 97201, United States
Get access

Abstract

We describe highly sensitive, non–invasive, label-free, electrical detection of protein biomarkers using nanoporous alumina membrane based electrochemical conductance based devices. The principle of operation of these sensors are based on electrochemical conductance varitions associated with the binding of antibody-antigen complexes to a metallic substrate.In these devices distinct pore clusters are selectively surface functionalized with specific antibodies, that are in turn are incorporated into micro scale arrays. Protein markers were routinely detected at nanomolar concentrations. This opens the potential for developing highly sensitive and selective biomarker detection assays in clinically relevant samples for diagnosis of complex disease state like vulnerable coronary plaque rupture that results in poor post surgical outcomes and other complex diseases.

Keywords

sensorsensorbiomedical
Type
Research Article
Information
MRS Online Proceedings Library (OPL) , Volume 926: Symposium CC – Electrobiological Interfaces on Soft Substrates , 2006 , 0926-CC03-02
Copyright
Copyright © Materials Research Society 2006

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

Abeloff, M.D., Armitage, J.O., Lichter, A.S. & Niederbuber, J.E. Clinical Oncology (Churchill Livingstone, New York, 2000).Google Scholar
Alivisatos, P., 2004 The use of nanocrystals in biological detection. Nat. Biotechnol. 22, 47–52.Google Scholar
Buffon, A., Biasucci, L.M., Liuzzo, G., D'Onofrio, G., Crea, F., and Maseri, A. (2002) Widespread coronary inflammation in unstable angina. N. Engl. J. Med. 347(1), 5–12.Google Scholar
Calvert, P., Cesarano, J., Chandra, H., Denham, H., Kasichainula, S., Vaidyanathan, R., Toughness in synthetic and biological multilayered systems. Philos. Transact. A. Math. Phys. Eng. Sci. 360(1791), 199–209.Google Scholar
Chen, R.J. et al. 2003 Non-covalent functionalization of carbon nanotubes for highly specific electronic biosensors. Proc. Natl. Acad. Sci. USA 100, 4984–4989.Google Scholar
Chou, S.F., Hsu, W.L., Hwang, J.M. and Chen, C.Y., 2004 Development of an immunosensor for human ferritin, a nonspecific tumor marker, based on surface plasmon resonance. Biosens. Bioelectron. 19, 999–1005.Google Scholar
Danesh, J., Collins, R, Appleby, P., and Peto, R. 1998 Association of fibrinogen, C-reactive protein, albumin, or leukocyte count with coronary heart disease: meta-analyses of prospective studies. Jama. 279(18), 1477–1482.Google Scholar
Etzioni, R. et al. 2003 The case for early detection. Nat. Rev. Cancer 3, 243–252 Google Scholar
Masuda, H., Yamada, H., Satoh, M., Asoh, H., Nakao, M., and Tamamura, T., 1997 Highly ordered nanochannel-array architecture in anodic alumina Appl. Phys. Lett. 71(19), 107–112 Google Scholar
Prasad, S., Zhang, X., Ozkan, C.S., Ozkan, M., 2004 Neuron-based microarray sensors for environmental sensing. Electrophoresis. 25(21-22), 3746–3760.Google Scholar
Prasad, S., Quijano, J., 2006 Development of nanostructured biomedical micro-drug testing device based on in-situ cellular activity monitoring. Biosens. Bioelectron. 21(7), 1219–1229 Google Scholar
Sander, C., 2000 Genomic medicine and the future of health care. Science 287, 1977–1978 Google Scholar
Schonbeck, U., and Libby, P., 2001 CD40 signaling and plaque instability. Circ Res. 89(12), 1092–1103.Google Scholar
Suefuji, H., Ogawa, H., Yasue, H., et al. 1997 Increased plasma tissue factor levels in acute myocardial infarction. Am. Heart J. 134(2 Pt 1), 253–259.Google Scholar
Suzuki, T., Kohno, H., Hasegawa, A., et al. 2002 Diagnostic implications of circulating oxidized low density lipoprotein levels as a biochemical risk marker of coronary artery disease. Clin. Bioche. 35(5), 347–353.Google Scholar
Ward, A.M., Catto, J.W.F., and Hamdy, F.C., 2001 Prostate specific antigen: biology, biochemistry and available commercial assays. Ann. Clin. Biochem. 38, 633–651.Google Scholar
Wu, G. et al. 2001 Bioassay of prostate-specific antigen (PSA) using microcantilevers. Nat. Biotechnol. 19, 856–860 Google Scholar