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Multiplexed Electrical Detection of Single Viruses

Published online by Cambridge University Press:  01 February 2011

Gengfeng Zheng ,
Fernando Patolsky  and
Charles M. Lieber
Gengfeng Zheng
Affiliation:
Department of Chemistry and Chemical Biology, Division of Engineering and Applied Sciences, Harvard University, Cambridge, MA 02138
Fernando Patolsky
Affiliation:
Department of Chemistry and Chemical Biology, Division of Engineering and Applied Sciences, Harvard University, Cambridge, MA 02138
Charles M. Lieber
Affiliation:
Department of Chemistry and Chemical Biology, Division of Engineering and Applied Sciences, Harvard University, Cambridge, MA 02138
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Abstract

We report direct, real-time electrical detection of single virus particles with high selectivity using nanowire field effect transistors. Measurements made with nanowire arrays modified with antibodies for influenza A showed discrete conductance changes characteristic of binding and unbinding in the presence of influenza A but not paramyxovirus or adenovirus. Moreover, simultaneous electrical and optical measurements using fluorescently-labelled influenza A demonstrate conclusively that the conductance changes correspond to binding/unbinding of single viruses at the surface of nanowire devices. In addition, studies of nanowire devices modified with antibodies specific for either influenza or adenovirus show that multiple viruses can be selectively detected in parallel. The possibility of large scale integration of these nanowire devices suggests potential for simultaneous detection of a large number of distinct viral threats at the single virus level.

Type
Research Article
Information
MRS Online Proceedings Library (OPL) , Volume 828: Symposium A – Semiconductor Materials for Sensing , 2004 , A2.2
Copyright
Copyright © Materials Research Society 2005

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References

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