Hostname: page-component-586b7cd67f-tf8b9 Total loading time: 0 Render date: 2024-11-23T11:20:01.991Z Has data issue: false hasContentIssue false
  • English
  • Français

Multiple Phage-Based Magnetoelastic Biosensors System for the Detection of Salmonella typhimurium and Bacillus anthracis Spores

Published online by Cambridge University Press:  01 February 2011

S. Huang ,
S. Li ,
H. Yang ,
M. L. Johnson ,
Ramji S Lakshmanan ,
I.-H. Chen ,
V. A. Petrenko ,
J. M. Barbaree  and
B. A. Chin
S. Huang
Affiliation:
[email protected], Auburn University, Materials Engineering, Auburn, Alabama, United States
S. Li
Affiliation:
[email protected], Auburn University, Materials Engineering, Auburn, Alabama, United States
H. Yang
Affiliation:
[email protected], Auburn University, Materials Engineering, Auburn, Alabama, United States
M. L. Johnson
Affiliation:
[email protected], Auburn University, Materials Engineering, Auburn, Alabama, United States
Ramji S Lakshmanan
Affiliation:
[email protected], Auburn University, Materials Engineering, Auburn, Alabama, United States
I.-H. Chen
Affiliation:
[email protected], Auburn University, Materials Engineering, Auburn, Alabama, United States
V. A. Petrenko
Affiliation:
[email protected], Auburn University, Materials Engineering, Auburn, Alabama, United States
J. M. Barbaree
Affiliation:
[email protected], Auburn University, Materials Engineering, Auburn, Alabama, United States
B. A. Chin
Affiliation:
[email protected], Auburn University, Materials Engineering, Auburn, Alabama, United States
Get access

Abstract

This paper presents a multiple magnetoelastic (ME) biosensor system for in-situ detection of S. typhimurium and B. anthracis spores in a flowing bacterial/spore suspension (5 x 101 - 5 x 108 cfu/ml). The ME biosensor was formed by immobilizing filamentous phage (specific to each detection target) on the ME platforms. An alternating magnetic field was used to resonate the ME biosensor to determine its resonance frequency. When cells/spores are bound to a ME biosensor surface, the additional mass of the cells/spores causes a decrease in the resonance frequency of the biosensor. The detection system was composed of a control sensor, an E2 phage-based biosensor (specific to S. typhimurium) and a JRB7 phage-based biosensor (specific to B. anthracis spores). The frequency response curves of the ME biosensors as a function of exposure time were then measured and the detection limit of the ME biosensor was observed to be 5 x 103 cfu/ml. The results show that phage-based ME biosensors can detect multiple pathogens simultaneously and offer good performance, including good sensitivity and rapid detection.

Keywords

ferromagneticsensoracoustic
Type
Research Article
Information
MRS Online Proceedings Library (OPL) , Volume 1129: Symposium V – Materials and Devices for Smart Systems III , 2008 , 1129-V04-16
Copyright
Copyright © Materials Research Society 2009

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

REFERENCES

[1] Mead, P. S., Slutsker, L., Dietz, V., McCaig, L. F., Bresee, J. S., Sharpiro, C., Griffin, P. M., and Tauxe, R. V., “Food-related illness and death in the United States,” Emerging Infectious Diseases, vol. 5, pp. 607–625, 1999.Google Scholar
[2] Berkow, R., The Merck Manual of Diagnosis and Therapy 1992, 16th ed.: Merck Publishing Group, 1992.Google Scholar
[3] Huang, S., Hu, J., Wan, J., Johnson, M. L., Shu, H., and Chin, B. A., “The effect of annealing and gold deposition on the performance of magnetoelastic biosensors,” Materials Science and Engineering C, vol. 28 pp. 380–386, 2008.Google Scholar
[4] Huang, S., Yang, H., Lakshmanan, R. S., Johnson, M. L., Chen, I., Wan, J., Wikle, H. C., Petrenko, V. A., Barbaree, J. M., Cheng, Z. Y., and Chin, B. A., “The Effect of Salt and Phage Concentrations on the Binding Sensitivity of Magnetoelastic Biosensors for B. anthracis Detection,” Biotechnology and Bioengineering, in press, 2008.Google Scholar
[5] Liang, C., Morshed, S., and Prorok, B. C., “Correction for longitudinal mode vibration in thin slender beams,” Applied Physics Letter., vol. 90, p. 221912, 2007.Google Scholar
[6] Stoyanov, P. G. and Grimes, C. A., “A remote query magnetostrictive viscosity sensor,” Sens. Actuators, vol. 80, pp. 8–l14, 2000.Google Scholar
[7] Petrenko, V. A. and Smith, G.P., “Phages from landscape libraries as substitue antibodies,” Protein Engineering, vol. 13, pp. 589–592, 2000.Google Scholar
[8] Petrenko, V. A., Smith, G. P., Gong, X., and Quinn, T., “A library of organic landscapes on filamentous phage,” Protein Engineering, vol. 9, pp. 797–801, 1996.Google Scholar