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Monoclonal Antibodies as New Macromolecular Hosts for Porphyrins

Published online by Cambridge University Press:  15 February 2011

Akira Harada ,
Kazutake Okamoto ,
Hiroyuki Fukushima ,
Keizou Shiotsuki  and
Mikiharu Kamachi
Akira Harada
Affiliation:
Department of Macromolecular Science, Faculty of Science, Osaka University, Toyonaka, Osaka 560, Japan
Kazutake Okamoto
Affiliation:
Department of Macromolecular Science, Faculty of Science, Osaka University, Toyonaka, Osaka 560, Japan
Hiroyuki Fukushima
Affiliation:
Department of Macromolecular Science, Faculty of Science, Osaka University, Toyonaka, Osaka 560, Japan
Keizou Shiotsuki
Affiliation:
Department of Macromolecular Science, Faculty of Science, Osaka University, Toyonaka, Osaka 560, Japan
Mikiharu Kamachi
Affiliation:
Department of Macromolecular Science, Faculty of Science, Osaka University, Toyonaka, Osaka 560, Japan
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Abstract

Monoclonal antibodies against meso-tetrakis(4-carboxyphenyl porphine)(TCPP) were prepared by immunizing Balb/c mice with TCPP bound keyhole limpet hemocyanin covalently and fusing the spleen cells with myeloma cells by using poly(ethylene glycol). The antibodies bind TCPP strongly with the dissociation constants of 10−6−2.5 × 10−7 M. One of the antibodies causes a large shift of the Soret band of TCPP to a longer wavelength and large induced Cotton effects(ICD) on TCPP. Quantitative analyses of the binding of TCPP to the antibody by ICD show that not only one-to-one binding but two-to one(binding site: TCPP) binding occurs in excess of the antibody over TCPP.

Type
Research Article
Information
MRS Online Proceedings Library (OPL) , Volume 277: Symposium V – Macromolecular Host-Guest Complexes: Optical, Optoelectronic, and Photorefractive Properties and Applications , 1992 , 87
Copyright
Copyright © Materials Research Society 1992

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References

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