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Miniaturized Silicon Apertures for Lipid Bilayer Reconstitution Experiments

Published online by Cambridge University Press:  31 January 2011

Michael Goryll  and
Nipun Chaplot
Michael Goryll
Affiliation:
[email protected], Arizona State University, Electrical Engineering, Tempe, Arizona, United States
Nipun Chaplot
Affiliation:
[email protected], Arizona State University, Electrical Engineering, Tempe, Arizona, United States
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Abstract

Ion channels reconstituted into lipid bilayer membranes can be used as a very sensitive and selective platform for high-throughput drug screening applications. In order to employ suspended lipid bilayer membranes for these experiments in form of a “lab-on-a-chip” configuration, a robust and affordable platform is required. In our study, we investigated the feasibility of hosting lipid bilayer membranes across micron-size apertures ranging from 5 μm – 50 μm in silicon. On these substrates, lipid bilayers were formed and characterized concerning their seal resistance, capacitance and breakdown voltage. Seal resistance values of up to 60 GΩ could be achieved repeatedly on these substrates.

Keywords

biomedicalmicrostructurereactive ion etching
Type
Research Article
Information
MRS Online Proceedings Library (OPL) , Volume 1191: Symposium OO – Materials and Strategies for Lab-on-a-Chip–Biological Analysis, Cell-Material Interfaces and Fluidic Assembly of Nanostructures , 2009 , 1191-OO08-05
Copyright
Copyright © Materials Research Society 2009

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References

[1] Klemic, K.G., Klemic, J.F., Reed, M.A., and Sigworth, F.J., Micromolded PDMS planar electrode allows patch clamp electrical recordings from cells. Biosensors & Bioelectronics 17(6-7), 597–604 (2002).10.1016/S0956-5663(02)00015-5Google Scholar
[2] Fertig, N., Meyer, Ch., Blick, R.H., Trautmann, Ch., and Behrends, J.C., Microstructured Glass Chip for Ion Channel Electrophysiology. Physical Review E 64, R40901–1 (2002).Google Scholar
[3] Wilk, S.J., Petrossian, L., Goryll, M., Thornton, T.J., Goodnick, S.M., Tang, J.M., and Eisenberg, R.S., Integrated electrodes on a silicon based ion channel measurement platform. Biosensors & Bioelectronics 23, 183–190 (2007).10.1016/j.bios.2007.03.030Google Scholar
[4] Mayer, M., Kriebel, J.K., Tosteson, M.T., and Whitesides, G.M., Microfabricated Teflon Membranes for Low-Noise Recordings of Ion Channels in Planar Lipid Bilayers. Biophysical Journal 85, 2684–2695 (2003).10.1016/S0006-3495(03)74691-8Google Scholar
[5] Mager, M.D., and Melosh, N.A., Lipid bilayer deposition and patterning via air bubble collapse. Langmuir 23(18), 9369–9377 (2007).10.1021/la701372bGoogle Scholar
[6] Levis, R.A., and Rae, J.L., Constructing a patch clamp setup. Methods Enzymol. 207, 14–66 (1992).10.1016/0076-6879(92)07004-8Google Scholar