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Measurement of Forces Generated by Chemomechanical Protein Aggregates Using Polymer BioMEMS

Published online by Cambridge University Press:  01 February 2011

Nicholas Ferrell ,
Stefan Schwan ,
Uwe Spohn ,
Andreas Heilmann  and
Derek Hansford
Nicholas Ferrell
Affiliation:
[email protected], Ohio State University, Department of Biomedical Engineering, 1080 Carmack Rd., 270 Bevis Hall, Columbus, OH, 43210, United States
Stefan Schwan
Affiliation:
[email protected], Fraunhofer Institute for Mechanics of Materials Halle, Biological Materials and Interfaces, Walter-Hülse-Strasse 1, Halle / Saale, 06120, Germany
Uwe Spohn
Affiliation:
[email protected], Fraunhofer Institute for Mechanics of Materials Halle, Biological Materials and Interfaces, Walter-Hülse-Strasse 1, Halle / Saale, 06120, Germany
Andreas Heilmann
Affiliation:
[email protected], Fraunhofer Institute for Mechanics of Materials Halle, Biological Materials and Interfaces, Walter-Hülse-Strasse 1, Halle / Saale, 06120, Germany
Derek Hansford
Affiliation:
[email protected], Ohio State University, Department of Biomedical Engineering, 1080 Carmack Rd., 270 Bevis Hall, Columbus, OH, 43210, United States
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Abstract

P-protein bodies (forisomes), in phloem cells of legumes, transform the chemical free energy of their reaction with alkaline earth metal ions into mechanical energy. In addition to using the bending of glass fibers, micromechanical forces generated by the switching of forisomes in aqueous solutions were measured by monitoring the bending of microscale polymer cantilever beams. The forisomes were fixed by chemisorption to the tips of four orthogonal beams of a BioMEMS microsystem designed and manufactured using the process of sacrificial layer micromolding. The sensor layout allows force measurements in the longitudinal and radial direction of the forisomes. For different forisomes a longitudinal force in the range from 84-136 nN and a radial force of 22-61 nN were measured.

Keywords

biomaterialmicroelectro-mechanical (MEMS)cellular (material form)
Type
Research Article
Information
MRS Online Proceedings Library (OPL) , Volume 1096: Symposium FF – Molecular Motors, Nanomachines and Active Nanostructures , 2008 , 1096-FF02-06
Copyright
Copyright © Materials Research Society 2008

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