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Materials Designed to Control and Examine The Function of Single Cells

Published online by Cambridge University Press:  15 February 2011

C.H. Thomas ,
J.B. Lhoest ,
D.G. Castner ,
C.D. Mcfarland  and
K.E. Healy
C.H. Thomas
Affiliation:
University, Depts. of Biological Materials and Biomedical Engineering, 311 E. Chicago Ave., Chicago, IL 60611
J.B. Lhoest
Affiliation:
NESAC/ Bio, University of Washington, Departments of Chemical Engineering and Bioengineering, Box 351750, Seattle, WA 98195
D.G. Castner
Affiliation:
NESAC/ Bio, University of Washington, Departments of Chemical Engineering and Bioengineering, Box 351750, Seattle, WA 98195
C.D. Mcfarland
Affiliation:
Department of Biomolecular Engineering, CSIRO, NSW, 2113, Australia
K.E. Healy
Affiliation:
University, Depts. of Biological Materials and Biomedical Engineering, 311 E. Chicago Ave., Chicago, IL 60611e-mail:[email protected]
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Abstract

Stress levels imparted on a cell have been shown to alter cell organization and function, presumably as a result of morphological cues affecting cytoskeletal organization. Materials with spatially resolved surface chemistry were designed to isolate individual mammalian cells to determine the influence of projected area on cell proliferation and cytoskeletal organization. Surfaces were fabricated using a photolithographic process resulting in islands of cell binding N-(2-aminoethyl)-3-aminopropyl-trimethoxysilane (EDS) separated by a non-adhesive interpenetrating polymer network [poly acrylamide-co-ethylene glycol; P(AAm-co-EG)]. The surfaces contained over 3800 adhesive islands/cm2, allowing for isolation of single cells with projected areas ranging from 100µm2to 10,000µm2. These surfaces provide a useful tool for researching how cell morphology and mechanical forces affect cell function.

Type
Research Article
Information
MRS Online Proceedings Library (OPL) , Volume 530: Symposium CC – Biomaterials Regulating Cell Function & Tissue Development , 1998 , 55
Copyright
Copyright © Materials Research Society 1998

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References

1 Folkman, J., and Moscona, A., Nature 273, 345 (1978).Google Scholar
2 Mooney, D., Hansen, L., Vacanti, J., Langer, R., Farmer, S., and Ingber, D., Journal of Cellular Physiology 151, 497 (1992).Google Scholar
3 Mooney, D. J., Langer, R., and Ingber, D. E., Journal of Cell Science 108, 2311 (1995).Google Scholar
4 Glowacki, J., Trepman, E., and Folkman, J., Proceedings of the Society for Experimental Biology and Medicine 172, 93 (1983).Google Scholar
5 Singhvi, R., Kumar, A., Lopez, G. P., Stephanopouios, G. N., Wang, D. I. C., Whitesides, G. M., and Ingbar, D. E., Science 264, 696 (1994).Google Scholar
6 Chen, C. S., Mrksich, M., Huang, S., Whitesides, G. M., and Ingber, D. E., Science 276, 1425 (1997).Google Scholar
7 Healy, K. E., Thomas, C. H., Rezania, A., Kim, J. E., McKeown, P. J., Lore, B., and Hockberger, P. E., Biomaterials 17, 95 (1996).Google Scholar
8 Thomas, C. H., McFarland, C. D., Jenkins, M. L., Rezania, A., Steele, J. G., and Healy, K. E., Journal of Biomedical Materials Research 37, 81 (1997).Google Scholar
9 Thomas, C. H., Lhoest, J. B., Castner, D. G., McFarland, C. D., and Healy, K. E., Journal of Biomechanical Engineering, Submitted, (1998).Google Scholar
10 Bearinger, J. P., Castner, D. G., Golledge, S. L., Rezania, A., Hubchak, S., and Helay, K. E., Langmnuir 13, 5175 (1997).Google Scholar
11 Bearinger, J. P., Castner, D. G., and Healy, K. E., Journal of Biomaterial Science: Polymer Edition 1 (1998).Google Scholar
12 McFarland, C. D., Thomas, C. H., Defilippis, C., Steele, J. G., and Healy, K. E., Journal of Biomedical Materials Research, In preparation, (1998).Google Scholar
13 Whitson, S. W., Whitson, M. A., Bowers, D. E., and Falk, M. C., Journal of Bone and Mineral Research 7, 727 (1992).Google Scholar