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Lubricin as a Novel Protein Coating to Prevent Bacterial Biofouling

Published online by Cambridge University Press:  23 October 2012

George E. Aninwene II ,
Erik Taylor ,
Douglas Hall ,
Amy Mei ,
Gregory D. Jay  and
Thomas J. Webster
George E. Aninwene II
Affiliation:
School of Engineering, Brown University, Providence, R I 02912, USA
Erik Taylor
Affiliation:
School of Engineering, Brown University, Providence, R I 02912, USA
Douglas Hall
Affiliation:
School of Engineering, Brown University, Providence, R I 02912, USA
Amy Mei
Affiliation:
School of Engineering, Brown University, Providence, R I 02912, USA
Gregory D. Jay
Affiliation:
Department of Medicine, Section of Emergency Medicine, Brown University, School of Medicine Providence, R I 02912,USA
Thomas J. Webster
Affiliation:
School of Engineering, Brown University, Providence, R I 02912, USA Department of Orthopaedics, Brown University, Providence, RI 0291, USA
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Abstract

Yearly, there are over six million cataract surgeries worldwide that involve intraocular lenses (IOLs) [1]. However, preventing post-operative biofouling of these lenses remains a challenge. One major complication is post-operative bacterial infection [2]. Surface modification of IOLs may provide a solution. This study proposes the use of the anti-adhesive protein lubricin (LUB), a glycoprotein found in the synovial fluid, as a means to make polymer surfaces less prone to bacterial adhesion and proliferation, thus reducing the opportunity for post-operative infection [3]. This study used extended bacteria growth trials in the presence of lubricin, vitronectin, and mucin to investigate how lubricin and protein sub-regions of lubricin reduce bacterial functions. This study showed for the first time that polymer surface coatings of lubricin and vitronectin significantly reduce Staphylococcus aureus growth over the course of 15 hours, while mucin was only able to delay the start of the Staphylococcus aureus exponential growth phase and retard proliferation. In solution, both lubricin and mucin significantly reduce bacterial proliferation. Thus, the results of this study demonstrated that lubricin and its sub-regions mucin and vitronectin should be studied for a wide range of antibacterial applications.

Keywords

adhesionbiomedicalbiological
Type
Articles
Information
MRS Online Proceedings Library (OPL) , Volume 1417: Symposium KK – Biomaterials for Tissue Regeneration , 2012 , mrsf11-1417-kk03-34
Copyright
Copyright © Materials Research Society 2012

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