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Loss of Infrared Linear Dichroism in Collagen Fibers as a Measure of Deterioration in Skin and Semi-Tanned Leather Artifacts

Published online by Cambridge University Press:  28 February 2011

Gregory S. Young
Gregory S. Young*
Affiliation:
Canadian Conservation Institute, Department of Communications, Ottawa, Canada K1A-0C8
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Abstract

Polarized radiation is used in a Fourier transform infrared (FTIR) microspectroscopic technique to detect molecular conformational change in collagen fibers resulting from deterioration. Absorbance spectra of single fibers are produced with polarization oriented first parallel (A‖) and then perpendicular (A⊥) to the fiber axis. The amide Ill band (1230- 1300 cm−1) shows strong positive dichroism, with up to six times greater absorbance occurring with parallel polarization. Losses of dichroism result from breakdown in the molecular structure of collagen. Integrations of the amide III bands are used in ratios, A‖/A⊥;, which provide a measure of the degree of molecular order. Ratios for degraded and undegraded material are compared to produce semi-quantitative information about deterioration.

Type
Research Article
Information
MRS Online Proceedings Library (OPL) , Volume 267: Symposium J – Materials Issues in Art and Archaeology III , 1992 , 859
Copyright
Copyright © Materials Research Society 1992

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References

1. Fraser, R.D.B., J. Mol. Biol. 193 115 (1987).CrossRefGoogle Scholar
2. Fraser, R.D.B. and Macrae, T.P., in Conformation in Fibrous Proteins,Academic Press, London (1973) pp. 344–402.Google Scholar
3. Fraser, R.D.B. and Macrae, T.P., in Conformation in Fibrous Proteins,Academic Press, London (1973) pp. 94–125.Google Scholar
4. Hukins, D.W.L., in Connective Tissue Matrix, Mac Millan Press Ltd.,London (1984) pp. 211–240.Google Scholar
5. Elliott, A., Infra-red Soectra and Structure of Organic Long-chainPolymers, London: Edward Arnold Ltd. (1969) 119pp.Google Scholar
6. Colthup, N.B., Daly, L.H. and.E. Wiberley Introduction to Infrared and Raman Spectroscopy, Academic Press, New York (1990) pp. 75–107.Google Scholar
7. Campbell, I.D., and Dwek, R.A., Biological Spectroscopy,Benjamin/Cummings Publishing Company, London (1984) pp. 52–56.Google Scholar
8. Siesler, H.W., and K. Holland-Mortiz, Infrared and Raman Spectroscopy ofPolymers, Marcel Dekker Inc., New York (1980) pp. 228–232.Google Scholar
9. Bresee, R.R., J.A.I.C. 25 39 (1986).Google Scholar
10. Struik, L.C.E., Physical Ageing in Amorphous Polymers and OtherMaterials, Elsevier, New York (1978).Google Scholar
11. Grattan, D.W., J.I.I.C.--C.G. 4 17 (1978).Google Scholar
12. Bowes, J.H., and Raistrick, A.S., J.A.L.C.A. 61 240 (1966).Google Scholar
13. Horie, C.V., Polym. Degradat. Stabil. 29 109 (1990).Google Scholar
14. Young, G.S., in Preprints of 9th Triennial Meeting, ICOM Committee forConservation, Dresden (1990) p. 626.Google Scholar
15. Young, G.S., Thermal analysis of untanned and semi-tanned hide (forthcoming).Google Scholar
16. Miles, C., Conservation Research Services Project #33, Canadian Conservation Institute, Department of Communicantions, Ottawa (1985).Google Scholar
17. Marcott, C.I., Noda, I. and Dowrey, A.E., Anal. Chimica Acta 250 131 (1991).CrossRefGoogle Scholar
18. Mantsch, H.H., Casal, H.L. and Jones, R.N., in Spectroscopy of BiologicalSystems, edited by Clark, R.J.H. and Hester, R.E., John Wiley and SonsLtd., New York (1986) pp. 1–46.Google Scholar
19. Levy, F., Anal. Chem. 60 1623 (1988).Google Scholar
20. Messerschmidt, R.G., in Microbeam Analysis--1987, 4B OoticalSpectroscopy: Micro-IR, edited by Geiss, R.H., San Francisco Press, San Francisco (1987) pp. 169–172.Google Scholar
21. Reffner, J.A., Coates, J.P. and Messerschmidt, R.G., American Laboratory April 105 (1987).Google Scholar
22. Chase, B., in Infrared Microspectroscopy: Theory and Applications, editedby Messerschmidt, R.G. and Harthcock, M.A., Marcel Dekker, Inc., New York (1988) pp. 93–102.Google Scholar
23. Badger, R.M. and Pullin, A.D.E., J. Chem. Phys. 22 1142 (1954).Google Scholar
24. Griffiths, R.P., and H.A. De Haseth, Fourier Transform Infrared Spectroscopy, New York: Wiley (1986).Google Scholar
25. Moffatt, D., (personal communication).Google Scholar
26. Bradbury, E.M., Burge, R.E., Randall, J.T. and Wilkinson, G.R., Discuss.Farad. Soc. 25 173 (1958).Google Scholar