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Local Environment of Surface-Polyelectrolyte-Bound DNA Oligomers

Published online by Cambridge University Press:  21 March 2011

Sangmin Jeon ,
Sung Chul Bae ,
Jiang John Zhao  and
Steve Granick
Sangmin Jeon
Affiliation:
Department of Materials Science and EngineeringUniversity of Illinois, Urbana, IL 61801
Sung Chul Bae
Affiliation:
Department of Materials Science and EngineeringUniversity of Illinois, Urbana, IL 61801
Jiang John Zhao
Affiliation:
Department of Materials Science and EngineeringUniversity of Illinois, Urbana, IL 61801
Steve Granick
Affiliation:
Department of Materials Science and EngineeringUniversity of Illinois, Urbana, IL 61801
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Abstract

Two-photon time-resolved fluorescence anisotropy methods were used to study the dynamical environment when fluorescent-labelled DNA oligomers (labelled with FAM, 6-fluorescein-6-carboxamido hexanoate) formed surface complexes with quaternized polyvinylpyridine (QPVP) cationic layers on a glass surface. We compared the anisotropy decay of DNA in bulk aqueous solution, DNA adsorbed onto QPVP, and QPVP-DNA-QPVP sandwich structures. When DNA was adsorbed onto QPVP, its anisotropy decay was dramatically retarded compared to the bulk, which means it had very slow rotational motion on the surface. Motions slowed down with increasing salt concentration up to a level of 0.1 M NaCl, but mobility began to increase at still higher salt concentration owing to detachment from the surface-immobilizing QPVP layers.

Type
Research Article
Information
MRS Online Proceedings Library (OPL) , Volume 651: Symposium T – Dynamics in Small Confining Systems V , 2000 , T4.2b.1
Copyright
Copyright © Materials Research Society 2001

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