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Investigation of biocompatibility on nitrogen-doped a-C:H film coating scaffold surface in in-vivo and in-vitro tests

Published online by Cambridge University Press:  14 January 2013

Yasuharu Ohgoe ,
Tomoaki Wada ,
Yasuyuki Shiraishi ,
Hidekazu Miura ,
Kenji K. Hirakuri ,
Akio Funakubo ,
Tomoyuki Yambe  and
Yasuhiro Fukui
Yasuharu Ohgoe
Affiliation:
Division of Electronic and Mechanical Engineering, Tokyo Denki University, Ishizaka, Hatoyama, Saitama, 350-0394Japan
Tomoaki Wada
Affiliation:
Division of Electronic and Mechanical Engineering, Tokyo Denki University, Ishizaka, Hatoyama, Saitama, 350-0394Japan
Yasuyuki Shiraishi
Affiliation:
Institute of Development, Aging and Cancer, Tohoku University, Seiryo-machi 4-1, Aoba-ku Sendai, 980-8575Japan
Hidekazu Miura
Affiliation:
Institute of Development, Aging and Cancer, Tohoku University, Seiryo-machi 4-1, Aoba-ku Sendai, 980-8575Japan
Kenji K. Hirakuri
Affiliation:
Department of Electrical Engineering, Tokyo Denki University, Senju Asahi-cho 5, Adachi-ku, Tokyo 120-8551Japan
Akio Funakubo
Affiliation:
Division of Electronic and Mechanical Engineering, Tokyo Denki University, Ishizaka, Hatoyama, Saitama, 350-0394Japan
Tomoyuki Yambe
Affiliation:
Institute of Development, Aging and Cancer, Tohoku University, Seiryo-machi 4-1, Aoba-ku Sendai, 980-8575Japan
Yasuhiro Fukui
Affiliation:
Division of Electronic and Mechanical Engineering, Tokyo Denki University, Ishizaka, Hatoyama, Saitama, 350-0394Japan
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Abstract

In this study, in order to investigate biocompatibility of nitrogen-doped hydrogenated amorphous carbon (a-C:H:N) film coating segmented polyurethane (SPU) scaffold fiber sheet (a-C:H:N-Scaffold) in in-vitro test, mouse fibroblasts (NIH 3T3) cells were grown on the a-C:H:N-Scaffold. The cell behavior was monitored by time-lapse imaging system. Additionally, the a-C:H:N-Scaffold was implanted at partial aorta descendens of a goat for 35 days. The surface morphology, composition, and wettability of the a-C:H:N-scaffold was estimated by Scanning Electron Microscope (SEM), X-ray photoelectron spectrometer (XPS), and contact angle measurement. In in-vitro test, it was observed that a-C:H:N film coating had a facilitatory effect on cell motility and cell growth. In in-vivo test, it was observed that the a-C:H:N-Scaffold surface was uniformly covered by neointima. The a-C:H:N-Scaffold surface had no thrombus formation as an inflammatory reaction and it was shown that the a-C:H:N film coating had a good blood compatibility. These results suggest that a-C:H:N film coating has good cytocompatibility and blood compatibility and it is a promising approach for improvement of biocompatibility of biomaterial surfaces.

Keywords

biomaterialcellular (material form)physical vapor deposition (PVD)
Type
Articles
Information
MRS Online Proceedings Library (OPL) , Volume 1498: Symposium L – Biomimetic, Bio-inspired and Self-Assembled Materials for Engineered Surfaces and Applications , 2013 , pp. 103 - 108
Copyright
Copyright © Materials Research Society 2013 

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