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Intrasectoral Zoning of Proteins and Nucleotides in Simple Crystalline Hosts

Published online by Cambridge University Press:  14 March 2011

Miki Kurimoto ,
Loyd D. Bastin ,
Daniel Fredrickson ,
Pamela N. Gustafson ,
Sei-Hum Jang ,
Werner Kaminsky ,
Scott Lovell ,
Christine A. Mitchell ,
Jean Chmielewski  and
Bart Kahr
Miki Kurimoto
Affiliation:
Department of Chemistry, Box 351700, University of Washington, Seattle, WA 98195-1700, U.S.A
Loyd D. Bastin
Affiliation:
Department of Chemistry, Box 351700, University of Washington, Seattle, WA 98195-1700, U.S.A
Daniel Fredrickson
Affiliation:
Department of Chemistry, Box 351700, University of Washington, Seattle, WA 98195-1700, U.S.A
Pamela N. Gustafson
Affiliation:
Department of Chemistry, Box 351700, University of Washington, Seattle, WA 98195-1700, U.S.A
Sei-Hum Jang
Affiliation:
Department of Chemistry, Box 351700, University of Washington, Seattle, WA 98195-1700, U.S.A
Werner Kaminsky
Affiliation:
Department of Chemistry, Box 351700, University of Washington, Seattle, WA 98195-1700, U.S.A
Scott Lovell
Affiliation:
Department of Chemistry, Box 351700, University of Washington, Seattle, WA 98195-1700, U.S.A
Christine A. Mitchell
Affiliation:
Department of Chemistry, Box 351700, University of Washington, Seattle, WA 98195-1700, U.S.A
Jean Chmielewski
Affiliation:
Department of Chemistry, 1393 Brown Laboratories, Purdue University, West Lafayette, IN 47907-1393, U.S.A
Bart Kahr
Affiliation:
Department of Chemistry, Box 351700, University of Washington, Seattle, WA 98195-1700, U.S.A
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Abstract

Oriented gases of biopolymers in simple, single crystal hosts might be used to measure anisotropic molecular properties of analytes that could not otherwise be crystallized. Here we show two types of crystals as examples of the single crystal matrix isolation of biopolymers: green fluorescent protein in α-lactose monohydrate as a model system for studying the kinetic stabilization of biopharmaceuticals, and adenosine phosphates in potassium dihydrogen phosphate, a first step in the matrix isolation of oligonucleotides. In each case, the hosts undergo compositional zoning – both intersectoral and intrasectoral – during growth from solution. Intrasectoral zoning is evident by the selective luminescence of adjacent vicinal slopes of growth active hillocks. Nucleotides furthermore distinguish between symmetry related growth sectors enantioselectively.

Type
Research Article
Information
MRS Online Proceedings Library (OPL) , Volume 620: Symposium M – Morphology & Dynamics of Crystal Surfaces in Complex… , 2000 , M9.8.1
Copyright
Copyright © Materials Research Society 2000

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