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Injection Molding of ProNectin®F Dispersed in Polystyrene for The Fabrication of Plastic Ware Activated Towards Attachment of Mammalian Cells

Published online by Cambridge University Press:  15 February 2011

Erwin R. Stedronsky ,
Joseph Cappello ,
Samuel David ,
David M. Donofrio ,
Tina Mcarthur ,
Kevin Mcgrath ,
Marilyn A. Panaro ,
Donna Putnam ,
William Spencer  and
Owens Wallis
Erwin R. Stedronsky
Affiliation:
Protein Polymer TECHNOLOGIES, 10655 Sorrento Valley Road, San Diego, CA 92121
Joseph Cappello
Affiliation:
Protein Polymer TECHNOLOGIES, 10655 Sorrento Valley Road, San Diego, CA 92121
Samuel David
Affiliation:
Protein Polymer TECHNOLOGIES, 10655 Sorrento Valley Road, San Diego, CA 92121
David M. Donofrio
Affiliation:
Protein Polymer TECHNOLOGIES, 10655 Sorrento Valley Road, San Diego, CA 92121
Tina Mcarthur
Affiliation:
Protein Polymer TECHNOLOGIES, 10655 Sorrento Valley Road, San Diego, CA 92121
Kevin Mcgrath
Affiliation:
Protein Polymer TECHNOLOGIES, 10655 Sorrento Valley Road, San Diego, CA 92121
Marilyn A. Panaro
Affiliation:
Protein Polymer TECHNOLOGIES, 10655 Sorrento Valley Road, San Diego, CA 92121
Donna Putnam
Affiliation:
Protein Polymer TECHNOLOGIES, 10655 Sorrento Valley Road, San Diego, CA 92121
William Spencer
Affiliation:
Protein Polymer TECHNOLOGIES, 10655 Sorrento Valley Road, San Diego, CA 92121
Owens Wallis
Affiliation:
Protein Polymer TECHNOLOGIES, 10655 Sorrento Valley Road, San Diego, CA 92121
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Abstract

ProNectin®F is a recombinant engineered protein polymer of de novo design which incorporates the RGD epitope recognized by mammalian cell integrins. It is biologically active as a cell attachment protein, manifests properties of a planar polymeric surfactant, and is extremely resistant to thermal degradation. ProNectin®F was dispersed onto polystyrene powder, fabricated into plastic ware through injection molding, and the plastic ware was shown to have cell attachment activity. This technology represents a new paradigm for the production of plastic ware useful for mammalian cell culture under serum free conditions; and more generally, for the production of molded devices for use in contact with cells in vitro or in vivo.

Type
Research Article
Information
MRS Online Proceedings Library (OPL) , Volume 330: Symposium S – Biomolecular Materials by Design , 1993 , 157
Copyright
Copyright © Materials Research Society 1994

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References

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