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In Vivo Interactions with (Tissue Culture Pretreated) Dermal Sheep Collagen

Published online by Cambridge University Press:  15 February 2011

P. B. van Wachem ,
P. B. van Wachem ,
L. H. H. Olde Damink ,
P. J. Dijkstra ,
J. Feijen  and
P. Nieuwenhuis
P. B. van Wachem
Affiliation:
Department of Histology and Cell Biology, Section Biomaterials Research, University of Groningen, Oostersingel 69/2, 9713 EZ Groningen, The Netherlands.
P. B. van Wachem
Affiliation:
Department of Histology and Cell Biology, Section Biomaterials Research, University of Groningen, Oostersingel 69/2, 9713 EZ Groningen, The Netherlands.
L. H. H. Olde Damink
Affiliation:
Department of Chemical Technology, University of Twente, p.o. Box 217, 7500 AE Enschede, The Netherlands
P. J. Dijkstra
Affiliation:
Department of Chemical Technology, University of Twente, p.o. Box 217, 7500 AE Enschede, The Netherlands
J. Feijen
Affiliation:
Department of Chemical Technology, University of Twente, p.o. Box 217, 7500 AE Enschede, The Netherlands
P. Nieuwenhuis
Affiliation:
Department of Histology and Cell Biology, Section Biomaterials Research, University of Groningen, Oostersingel 69/2, 9713 EZ Groningen, The Netherlands.
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Abstract

Pretreatment in tissue culture (TC) was previously found to markedly reduce the in vitro cytotoxicity of two types of crosslinked dermal sheep collagens (DSC's). This in vivo study confirms our in vitro results, in that TC-pretreatment of crosslinked DSC's resulted in the marked reduction or elimination of cytotoxic effects, such as increased cell infiltration, a deviant neutrophil-morphology, lipid formation and cell death. TC-pretreatment affected the crosslinked state of both DSC's in a different way, which could be deduced from the differences in gelatin-formation and presence of giant cells from macrophage- or fibroblast-origin. The results are explained in view of the differences in crosslinking.

Type
Research Article
Information
MRS Online Proceedings Library (OPL) , Volume 252: Symposium T – Tissue-Inducing Biomaterials , 1991 , 117
Copyright
Copyright © Materials Research Society 1992

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References

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