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In vitro Study of the Rheological Effect of Mixtures of Perfluorotributylamine (FC-43) and Stroma Free Hemoglobin (SFH) On Sickled Erythrocytes

Published online by Cambridge University Press:  26 February 2011

Carl A. Reindorf  and
Novelette E. Thompson
Carl A. Reindorf
Affiliation:
Center for Sickle Cell Disease, Howard University College of Medicine, Washington, D.C.
Novelette E. Thompson
Affiliation:
Center for Sickle Cell Disease, Howard University College of Medicine, Washington, D.C.
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Abstract

Stroma Free Hemoglobin (SFH) and Perfluorotributylamine (FC-43) have been extensively studied as oxygen carriers. Therefore, capitalizing on the role of oxygen reversing sickling, and thus decreasing resistance, this study was undertaken to investigate possible differences in the rheological effects of mixtures of FC-43 and SFH at different ratios. We used the positive pressure cell filtration system (PPCFS) of Lessin and Kurantsin- Mills. FC-43 was purchased from Alpha Therapeutics Corporation, and SFH was prepared by the method of Rabiner et al. The FC-43/SFH mixtures were made on a volume basis in ratios of 1:1 and 1:2. Fresh sickle cell anemia (HbSS) blood was used to prepare 0.2% cell suspensions which were deoxygenated, by bubbling with moist N2 for one hour. Studies of the rheological effects of FC-43/SFH mixtures on sickled erythrocytes were determined by obtaining filtration measurements on the following:- 1. sickle cells in ambient air; 2. deoxygenated sickle cells; 3. deoxygenated sickle cells + oxygenated FC-43/SFH (1:1 v/v); 4. deoxygenated sickle cells + oxygenated FC - 43/SFH (1:2 v/v). The results showed that treatment of deoxygenated sickle erythrocytes with oxygenated SFH/FC-43 mixtures decreased the flow resistance of the deoxygenated cells. Secondly, the SFH/FC-43 (1:2 v/v) mixture caused greater decrease in flow resistance than the 1:1 mixture.

Therefore it is reasonable to conclude that the mixtures of SFH/FC-43 (1:2 v/v) ratio appear to have a additive effect in reducing the flow resistance i.e. increasing the filterability of sickled erythrocytes.

Type
Research Article
Information
MRS Online Proceedings Library (OPL) , Volume 110: Symposium M – Biomedical Materials and Devices , 1987 , 119
Copyright
Copyright © Materials Research Society 1988

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