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Hydrophobicity and Biofilm Growth of E-coli RP437, MG1655 and B/r

Published online by Cambridge University Press:  17 October 2012

Li Hong Zhao
Affiliation:
Department of Physics and Astronomy, The University of North Carolina, Chapel Hill NC 27599
Pabitra N. Sen
Affiliation:
Department of Physics and Astronomy, The University of North Carolina, Chapel Hill NC 27599
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Abstract

Two strains of E-Coli K-12, viz, RP437, MG1655 and B/r (E. coli B derivative, not a K-12 strain) were grown on various surfaces to study bacterial adhesion and subsequent biofilm formation. We observed biofilm and large colonies on cover slides, beads made of soda lime or borosilicate glasses, on plasma treated PDMS (Polydimethylsiloxane), on Tissue Culture (TC) polystyrene, and observed some clusters on plasma treated ZnTi cover slide; but no evidence of biofilm on untreated-PDMS and ZnTi glass cover slides. From contact angle measurements, we conclude that the hydrophobic nature of untreated PDMS prevent bacterial adhesion for these three strains.

Type
Articles
Copyright
Copyright © Materials Research Society 2012

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References

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