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Human Bone Marrow Stem Cell Responses on Electrospun Bombyx mori Silk Fibroin

Published online by Cambridge University Press:  11 February 2011

Hyoung-Joon Jin ,
Jingsong Chen ,
Vassilis Karageorgiou ,
Gregory H. Altman  and
David L. Kaplan
Hyoung-Joon Jin
Affiliation:
Department of Chemical & Biological Engineering, Bioengineering Center Tufts University, 4 Colby Street, Medford, MA 02155, U.S.A.
Jingsong Chen
Affiliation:
Department of Chemical & Biological Engineering, Bioengineering Center Tufts University, 4 Colby Street, Medford, MA 02155, U.S.A.
Vassilis Karageorgiou
Affiliation:
Department of Chemical & Biological Engineering, Bioengineering Center Tufts University, 4 Colby Street, Medford, MA 02155, U.S.A.
Gregory H. Altman
Affiliation:
Department of Chemical & Biological Engineering, Bioengineering Center Tufts University, 4 Colby Street, Medford, MA 02155, U.S.A.
David L. Kaplan
Affiliation:
Department of Chemical & Biological Engineering, Bioengineering Center Tufts University, 4 Colby Street, Medford, MA 02155, U.S.A.
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Abstract

Electrospinning for the formation of nanoscale diameter fibers has been explored for high performance filters and biomaterial scaffolds for vascular grafts or wound dressings. Fibers with nanoscale diameters provide benefits due to high surface area. In this study we used electrospinning for protein-based biomaterials to fabricate scaffolds from aqueous regenerated silkworm silk, Bombyx mori,. Adhesion, spreading and proliferation of human bone marrow stem cells (hBMSCs) on electrospun silk was characterized. Scanning electron microscopy (SEM) and MTT analyses demonstrated that the electrospun silk matrices promoted hBMSC attachment and proliferation over 10 days in culture. The responses of the hBMSCs on the electrospun silk matrices, combined with the biocompatibile properties of the silk fibroin protein matrix, suggest potential for use of this biomaterial as scaffolds for tissue engineering.

Type
Research Article
Information
MRS Online Proceedings Library (OPL) , Volume 735: Symposium C – Bioinspired Nanoscale Hybrid Systems , 2002 , C11.12
Copyright
Copyright © Materials Research Society 2003

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References

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