Hostname: page-component-586b7cd67f-vdxz6 Total loading time: 0 Render date: 2024-11-25T15:16:46.006Z Has data issue: false hasContentIssue false
  • English
  • Français

Honeycomb Films of Biodegradable Polymers for Tissue Engineering

Published online by Cambridge University Press:  01 February 2011

Takehiro Nishikawa ,
Keiko Arai ,
Junko Hayashi ,
Masahiko Hara  and
Masatsugu Shimomura
Takehiro Nishikawa
Affiliation:
Spatio-Temporal Function Materials Research Group, The Institute of Physical and Chemical Research (RIKEN), Hirosawa 2-1, Wako, Saitama, 351-0198, JAPAN.
Keiko Arai
Affiliation:
Spatio-Temporal Function Materials Research Group, The Institute of Physical and Chemical Research (RIKEN), Hirosawa 2-1, Wako, Saitama, 351-0198, JAPAN.
Junko Hayashi
Affiliation:
Spatio-Temporal Function Materials Research Group, The Institute of Physical and Chemical Research (RIKEN), Hirosawa 2-1, Wako, Saitama, 351-0198, JAPAN.
Masahiko Hara
Affiliation:
Spatio-Temporal Function Materials Research Group, The Institute of Physical and Chemical Research (RIKEN), Hirosawa 2-1, Wako, Saitama, 351-0198, JAPAN.
Masatsugu Shimomura
Affiliation:
Spatio-Temporal Function Materials Research Group, The Institute of Physical and Chemical Research (RIKEN), Hirosawa 2-1, Wako, Saitama, 351-0198, JAPAN.
Get access

Abstract

We report that microporous films (honeycomb films) can lead various types of cells to tissue formation. The honeycomb films were fabricated by applying a moist air to a spread polymer solution containing biodegradable polymers (poly(L-lactic acid) (PLLA) and poly (ε-caprolactone) (PCL)) and an amphiphilic polymer. Hepatocytes were cultured on a self-supporting honeycomb film of PLLA. The hepatocytes formed a single layer of columnar shape cells with a thickness of 20 μm. The tissue formation of hepatocytes specifically occurred on the honeycomb film of PLLA, not on a flat film of PLLA. Three dimensional tissue structures were formed, when cells were cultured on both sides of the self-supporting honeycomb film. Double layers of hepatocytes were obtained by the method. Striated tissues such as heart and blood vessel could be reconstructed by utilizing a stretched honeycomb film of PCL.

Type
Research Article
Information
MRS Online Proceedings Library (OPL) , Volume 724: Symposium N – Biological and Biomimetic Materials Properties to Function , 2002 , N11.7
Copyright
Copyright © Materials Research Society 2002

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

1. Bell, E., “Organotypic and Histiotypic Models of Engineered Tissues,” Saltzmann, W., “Cell Interactions with Polymers,” Hubbel, J., “Matrix Effects,” Thomson, R., Shung, A., Yaszemski, M. and Mikos, A., “Polymer Scaffold Processing,” Principles of Tissue Engineering, ed. Lanza, R., Langer, R. and Vacanti, J. (Academic Press, 2000) pp.181–194, pp.221-236, pp.237-250, pp.251-262.Google Scholar
2. Dee, K., Puleo, D. and Bizios, R., Biomaterials Today 3, 7 (2000).Google Scholar
3. McClary, K. and Grainger, D., Biomaterials 20, 2435 (1999).Google Scholar
4. Mooney, D., Hansen, L., Vacanti, J., Langer, R., Farmer, S. and Ingber, D., J. Cell. Physiol. 151, 497 (1992).Google Scholar
5. Curtis, A. and Wilkinson, C., Biomaterials 18, 1573 (1997).Google Scholar
6. Maruyama, N., Koito, T., Nishida, J., Sawadaishi, T., Cieren, X., Ijiro, K., Karthaus, O. and Shimomura, M., Thin Solid Films 327-329, 854 (1998).Google Scholar
7. Seglen, P. O., Exper. Cell Res. 74, 450 (1972).Google Scholar
8. Denyer, M., Riehle, M., Hayashi, J., Scholl, M., Sproessler, C., Britland, S., Offenheusser, A. and Knoll, W., In Vitro Cell. Dev Biol.-Animal 35, 352 (1999).Google Scholar
9. Engelberg, I. and Kohn, J., Biomaterials 12, 292 (1991).Google Scholar
10. Chen, C., Mrksich, M., Huang, S., Whitesides, G. and Ingber, D., Science 276, 1425 (1997).Google Scholar