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Highly Sensitive Surface Plasmon Resonance Sensor on Nanoscale Bioactive Surfaces for Specific Detection of Tri-Nitro Toluene

Published online by Cambridge University Press:  01 February 2011

Praveen Singh ,
Takeshi Onodera ,
Kiyoshi Matsumoto ,
Norio Miura  and
Kiyoshi Toko
Praveen Singh
Affiliation:
[email protected], Kyushu University, Department of Electronics, 744 Motooka, Nishi-ku, R.No.459,W2, Fukuoka, 819-0395, Japan, 81-92-8023762, 81-92-8023770
Takeshi Onodera
Affiliation:
[email protected], Kyushu University, Department of Electronics, Graduate school of Information Science and Electrical Engineeting, 744 Motooka, Nishi-ku, Fukuoka, 819-0395, Japan
Kiyoshi Matsumoto
Affiliation:
[email protected], Kyushu University, Graduate School of Agriculture, 6-10-1 Hakozaki, Higashi-ku, Fukuoka, 812-8581, Japan
Norio Miura
Affiliation:
[email protected], Kyushu University, Art, Science and Technology Centre for Cooperative Research, Kasuga-shi, Fukuoka, 816-8580, Japan
Kiyoshi Toko
Affiliation:
[email protected], Kyushu University, Department of Electronics, Graduate School of Information Science and Electrical Engineering, 744 Motooka, Nishi-ku, Fukuoka, 819-0395, Japan
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Abstract

A nano-scale biosensor chip surface was fabricated using dinitro-phenylated key hole limpet (DNP-KLH) protein conjugate as ligand supported by underlying 11-amino 1-undecanethiol hydrochloride(AUT) self assembled monolayer (SAM) and bis sulfosuccinimidyl suberate(BS3) as crosslinker. Bioactive thin films were fabricated over gold chip via layer-by-layer self assembly methods. Biomolecular interaction between substrate specific anti-TNT antibody and DNP-KLH conjugate surface was monitored through surface plasmon resonance based optical sensor. The quantitation of tri-nitro toluene(TNT) on this bioactive surface was done using the solution based competitive inhibition assay. The DNP-KLH surface biosensor has shown a detection limit of 0.14 ng/ml(140 ppt) for TNT molecule. The detection limit of surface plasmon resonance(SPR) biosensor was further enhanced by using goat anti mouse antibody to the 0.002 ng/ml for TNT analyte. This TNT specific biosensor holds the promise to be one of most sensitive sensor surface under indirect competitive assay format. A short injection (12 sec) of 10 mM Glycine-HCl solution was found adequate for regeneration of DNP-KLH surface for repeated use.

Keywords

nanoscaleopticalbiological
Type
Research Article
Information
MRS Online Proceedings Library (OPL) , Volume 951: Symposium E – Nanofunctional Materials, Nanostructures and Novel Devices for Biological and Chemical Detection , 2006 , 0951-E07-12
Copyright
Copyright © Materials Research Society 2007

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References

REFERENCES

1. Cumming, C.J., Aker, C., Fisher, M., Fox, M., Grone, M.J.L., Reust, D., Rockly, M.G., Swager, T.M., Towers, E. and Williams, V., IEEE Trans. Geosc. Remote Sensing 39, 1119(2001).Google Scholar
2. Miura, N., Higobashi, H., Sakai, G., Takeyasu, A., Uda, T. and Yamazoe, N., Sens. Actuators B 13–14, 188(1993).Google Scholar
3. Yang, J.S. and Swager, T.M., J. Amer. Chem. Soc. 120, 11864(1998).Google Scholar
4. Thundat, T., SPIE's oemagzine February, 25–26(2005).Google Scholar
5. Rabbany, S.Y., Lane, W.J., Marganski, W.A., Kusterbeck, A.W. and Ligler, F.S., 2002. J. Immunol. Methods 246, 69(2002)Google Scholar
6. Naimushin, A.N., Soelberg, S.D., Nguyen, D.K., Dunlap, L., Barthollomew, D., Elkind, J., Melendez, J. and Furlong, C., 2002. Biosens. Bioelectron. 17, 573(2002).Google Scholar
7. Chinowsky, T. M., Quinn, J.G., Bartholomew, D.U., Kaiser, R., Elkind, J.L., 2003. Performance of the Spreeta 2000 intergrated surface plasmon resonance affinity sensor. Sens. Actuators B 6954, 1–9.Google Scholar
8. Homola, J., Yee, S. and Myszka, D., “Surface Plasmon Resonance Biosensors”, Ed. Ligler, S., Taitt, C.A.R., (Elsevier, 2002) The Netherlands, pp. 207–251.Google Scholar
9. Shankaran, D. Ravi, Gobi, K.V., Matsumoto, K., Imato, T., Toko, K. and Miura, N., 2004. Sens. Actuators B 100, 450(2004).Google Scholar
10. Matsumoto, K., Torimaru, A., Ishitobi, S., Sakai, T., Ishikawa, H., Toko, K., Miura, N. and Imato, T., Talanta 68, 305(2005).Google Scholar
11. Mark, S.S., Sandhyarani, N., Zhu, C., Campagnolo, C. and Batt, C.A., Langmuir 20, 6808(2004).Google Scholar