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Highly Efficient Detector of the Neurotransmitter ACh and AChE Inhibitors

Published online by Cambridge University Press:  15 March 2011

Ilya Goykhman ,
Nina Korbakov ,
Carmen Bartic ,
Gustaaf Borghs ,
Micha E. Spira ,
Josef Shappir  and
Shlomo Yitzchaik
Ilya Goykhman
Affiliation:
Department of Analytical and Inorganic Chemistry, The Hebrew University of Jerusalem, Jerusalem, 91904, Israel School of Engineering, The Hebrew University of Jerusalem, Jerusalem, 91904, Israel
Nina Korbakov
Affiliation:
Department of Analytical and Inorganic Chemistry, The Hebrew University of Jerusalem, Jerusalem, 91904, Israel
Carmen Bartic
Affiliation:
IMEC, Kapeldreef 75, Leuven, B-3001, Belgium
Gustaaf Borghs
Affiliation:
IMEC, Kapeldreef 75, Leuven, B-3001, Belgium
Micha E. Spira
Affiliation:
Department of Neurobiology, The Hebrew University of Jerusalem, Jerusalem, 91904, Israel
Josef Shappir
Affiliation:
School of Engineering, The Hebrew University of Jerusalem, Jerusalem, 91904, Israel
Shlomo Yitzchaik
Affiliation:
Department of Analytical and Inorganic Chemistry, The Hebrew University of Jerusalem, Jerusalem, 91904, Israel
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Abstract

Among the variety of applications for biosensorsone of the exciting frontiers is to utilize those devices as post-synaptic sensing elements in chemical coupling between neurons and solid-state systems. The first necessary step to attain this challenge is to realize highly efficient detector for neurotransmitter acetylcholine (ACh). Herein, we demonstrate that the combination of floating gate configuration of ion-sensitive field effect transistor (ISFET) together with diluted covalent anchoring of enzyme acetylcholinesterase (AChE) onto device sensing area reveals a remarkable improvement of a four orders of magnitude in dose response to ACh. This high range sensitivity in addition to the benefits of peculiar microelectronic design show, that the presented hybrid provides a competent platform for assembly of artificial chemical synapse junction. Furthermore, our system exhibits clear response to eserine, a competitive inhibitor of AChE, and therefore it can be implemented as an effective sensor of pharmacological reagents, organophosphates, and nerve gases as well.

Type
Research Article
Information
MRS Online Proceedings Library (OPL) , Volume 1009: Symposium U – Advanced Materials for Neuroprosthetic Interfaces , 2007 , 1009-U08-04
Copyright
Copyright © Materials Research Society 2007

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