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Grafted Poly(acrylic acid) Brushes for Cell-Surface Interactions

Published online by Cambridge University Press:  01 February 2011

Jöns Hilborn ,
B. Gupta ,
L. Garamszegi ,
A. Laurent ,
C.J.G. Plummer ,
I. Bisson ,
P. Frey  and
J.L. Hedrick
Jöns Hilborn
Affiliation:
Polymer Laboratory, Department of Material Science, Swiss Federal Institute of Technology, 1015 Lausanne, Switzerland, [email protected]
B. Gupta
Affiliation:
Polymer Laboratory, Department of Material Science, Swiss Federal Institute of Technology, 1015 Lausanne, Switzerland, [email protected]
L. Garamszegi
Affiliation:
Polymer Laboratory, Department of Material Science, Swiss Federal Institute of Technology, 1015 Lausanne, Switzerland, [email protected]
A. Laurent
Affiliation:
Polymer Laboratory, Department of Material Science, Swiss Federal Institute of Technology, 1015 Lausanne, Switzerland, [email protected]
C.J.G. Plummer
Affiliation:
Polymer Laboratory, Department of Material Science, Swiss Federal Institute of Technology, 1015 Lausanne, Switzerland, [email protected]
I. Bisson
Affiliation:
Pediatric Urology Resarch Laboratory, Centre Hospitalier Universitaire Vaudois, 1011 Lausanne, Switzerland
P. Frey
Affiliation:
Pediatric Urology Resarch Laboratory, Centre Hospitalier Universitaire Vaudois, 1011 Lausanne, Switzerland
J.L. Hedrick
Affiliation:
IBM Almaden Resarch Center, San Jose, CA 95120, USA
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Abstract

Poly(acrylic acid) (PAA) was grafted onto RF plasma treated PET films and onto SiO2 covered glass surfaces. PET films with various amounts of grafted PAA (0.4, 5, 11 and 19 μm/cm2) were immersed into a solution of collagen to allow for polyionic complex formation as a method for protein immobilization. It was found that the amount of complexed collagen was close to proportional to the amount of PAA. A closer investigation of the optical density of the PAA brushes close to the glass surface wa was performed using Reflectometric Inteference Spectroscopy. The supression of possibilities of extension and collaps of the brushes upon variation in pH is suggested to be caused by polyionic crosslinking between protonated collagen and deprotonated PAA. Such PAA-collagen surfaces with PAA concentrations lower that 10 μm/cm2 were shown to be suitable substrates for growth of human bladder smooth muscle cells.

Type
Research Article
Information
MRS Online Proceedings Library (OPL) , Volume 629: Symposium FF – Interfaces, Adhesion and Processing in Polymer Systems , 2000 , FF4.1
Copyright
Copyright © Materials Research Society 2000

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References

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