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Gold Nanoparticle Enlargement Coupled with Fluorescence Decrease for Highly Sensitive Detection of Analytes

Published online by Cambridge University Press:  28 January 2011

Seong Yoon Lim ,
Jae Hong Kim ,
Joon Seok Lee  and
Chan Beum Park
Seong Yoon Lim
Affiliation:
Department of Materials Science and Engineering, Korea Advanced Institute of Science and Technology 335 Science Road, Daejeon 305-701, Republic of Korea
Jae Hong Kim
Affiliation:
Department of Materials Science and Engineering, Korea Advanced Institute of Science and Technology 335 Science Road, Daejeon 305-701, Republic of Korea
Joon Seok Lee
Affiliation:
Department of Materials Science and Engineering, Korea Advanced Institute of Science and Technology 335 Science Road, Daejeon 305-701, Republic of Korea
Chan Beum Park
Affiliation:
Department of Materials Science and Engineering, Korea Advanced Institute of Science and Technology 335 Science Road, Daejeon 305-701, Republic of Korea
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Abstract

We present a versatile and facile route for highly sensitive detection of analytes through coupling the enlargement of gold nanoparticles (Au NPs) with fluorescence decrease. The fluorescence intensity of dye molecules (e.g., fluorescein or rhodamine B) significantly decreased with the increasing concentration of reducing agents, such as hydrogen peroxide and hydroquinone. The sensitivity for the detection of reducing agents was much higher than other detection methods based on the absorbance measurement of enlarged gold nanoparticles or quantum dot-enzyme hybridization. We could successfully detect acetylthiocholine with the detection limit of several nM orders, using an enzymatic reaction by acetylcholinesterase, a key route for the detection of toxic organophosphate compounds. The fluorescence decreasing approach described in this work requires only a simple addition of fluorescence dye to the reaction solution without any chemical modification. The strategy of fluorescence decrease coupled with nanoparticle growth will be applied on the fluorescent substrate to develop detection templates for highly sensitive optical biosensor.

Keywords

sensorAutransmission electron microscopy (TEM)
Type
Research Article
Information
MRS Online Proceedings Library (OPL) , Volume 1301: Symposium V/NN/OO/PP – Soft Matter, Biological Materials and Biomedical Materials—Synthesis, Characterization and Applications , 2011 , mrsf10-1301-pp05-02
Copyright
Copyright © Materials Research Society 2011

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References

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