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Glass Bone Implants: The Effect of Surface Topology on Attachment and Proliferation of Osteoblast Cells on 45S Bioactive Glass

Published online by Cambridge University Press:  31 January 2011

Raina Himani Jain ,
Shaojie Wang ,
Hassan M. Moawad ,
Matthias M. Falk  and
Himanshu Jain
Raina Himani Jain
Affiliation:
[email protected], Lehigh University, Biological Sciences, Bethlehem, Pennsylvania, United States
Shaojie Wang
Affiliation:
[email protected], Lehigh University, Materials Science and Engineering, Bethlehem, Pennsylvania, United States
Hassan M. Moawad
Affiliation:
[email protected], Lehigh University, International Materials Institute for New Functionality in Glass, Bethlehem, Pennsylvania, United States
Matthias M. Falk
Affiliation:
[email protected], Lehigh University, Biological Sciences, Bethlehem, Pennsylvania, United States
Himanshu Jain
Affiliation:
[email protected], Lehigh University, Materials Science and Engineering, Bethlehem, Pennsylvania, United States
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Abstract

Bioglass 45S is a promising bone implant material with superior biocompatibility. Past research showed that adhesion of bone cells to titanium is strongly affected by its surface architecture. However, little is known about the role of surface topology of glass on its use as an implant. Thus, we systematically investigated the effect of surface roughness (Ra ∼ 0.01 – 1.2 μm) on cell adhesion and proliferation on 45S Bioglass in vitro. MG63 osteosarcoma and MC3T3 osteoblast precursor cells were seeded on the glass samples, and incubated for up to 6 days. The attachment, morphology and proliferation of cells were investigated using fluorescence microscopy. Our results show that cell attachment (as indicated by cell spreading and number of focal adhesion sites), and proliferation rate decrease with increasing roughness of bioactive glass surface. These findings provide important insight for improving surface characteristics of bioactive glass bone implants.

Keywords

biomaterialboneamorphous
Type
Research Article
Information
MRS Online Proceedings Library (OPL) , Volume 1235: Symposium RR – Engineering Biomaterials for Regenerative Medicine , 2009 , 1235-RR03-47
Copyright
Copyright © Materials Research Society 2010

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