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Functionalized Waveguides for Biological Assays

Published online by Cambridge University Press:  01 February 2011

Aaron S. Anderson ,
Andrew M. Dattelbaum ,
Gabriel A. Montaño ,
Jurgen G. Schmidt ,
Jennifer S. Martinez ,
W. Kevin Grace ,
Karen M. Grace  and
Basil I. Swanson
Aaron S. Anderson
Affiliation:
Los Alamos National Lab, Center for Integrated Nanotechnologies, MS K771, Los Alamos National Lab, Los Alamos, NM, 87545, United States, 505-665-0142
Andrew M. Dattelbaum
Affiliation:
[email protected], Los Alamos National Laboratory, MS K771, Los Alamos National Lab, Los Alamos, NM, 87545, United States
Gabriel A. Montaño
Affiliation:
[email protected], Los Alamos National Laboratory, MS K771, Los Alamos National Lab, Los Alamos, NM, 87545, United States
Jurgen G. Schmidt
Affiliation:
[email protected], Los Alamos National Laboratory, MS K771, Los Alamos National Lab, Los Alamos, NM, 87545, United States
Jennifer S. Martinez
Affiliation:
[email protected], Los Alamos National Laboratory, MS K771, Los Alamos National Lab, Los Alamos, NM, 87545, United States
W. Kevin Grace
Affiliation:
[email protected], Los Alamos National Laboratory, MS K771, Los Alamos National Lab, Los Alamos, NM, 87545, United States
Karen M. Grace
Affiliation:
[email protected], Los Alamos National Laboratory, MS K771, Los Alamos National Lab, Los Alamos, NM, 87545, United States
Basil I. Swanson
Affiliation:
[email protected], Los Alamos National Laboratory, MS K771, Los Alamos National Lab, Los Alamos, NM, 87545, United States
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Abstract

We report here a procedure for the functioalization of SiO2-coated, SiONx waveguides for biological assays. Surface functionalization occurs by self-assembly of an amine-terminated silane monolayer on the waveguide, followed by partial chemical modification with functionalized polyethylene glycol (PEG) groups. Functionalized surfaces were characterized by atomic force microscopy and contact angle measurements. When combined with a BSA blocking step, these functional PEG surfaces significantly reduced non-specific binding and allowed for specific binding to occur. An antibody sandwich assay for detection of Bacillus anthracis protective antigen was used to validate these surfaces for sensing applications.

Keywords

sensorbiologicalself-assembly
Type
Research Article
Information
MRS Online Proceedings Library (OPL) , Volume 950: Symposium D – Biosurfaces and Biointerfaces , 2006 , 0950-D04-38
Copyright
Copyright © Materials Research Society 2007

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References

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