Hostname: page-component-cd9895bd7-jn8rn Total loading time: 0 Render date: 2024-12-27T02:04:49.423Z Has data issue: false hasContentIssue false
  • English
  • Français

Fatigue Microdamage in Bovine Cortical Bone Imaged by Micro-Computed Tomography Using a Barium Sulfate Contrast Agent

Published online by Cambridge University Press:  26 February 2011

Huijie Leng ,
Xiang Wang ,
Glen L Niebur  and
Ryan K Roeder
Huijie Leng
Affiliation:
[email protected], University of Notre Dame, 365 Fitzpatrick Hall, Notre Dame, IN, 46556, United States, 5746314381
Xiang Wang
Affiliation:
[email protected], United States
Glen L Niebur
Affiliation:
[email protected], United States
Ryan K Roeder
Affiliation:
[email protected], United States
Get access

Abstract

Accumulation of microdamage during fatigue can lead to increased fracture susceptibility in bone. Current techniques for imaging microdamage in bone are inherently destructive and two-dimensional. A non-destructive, three-dimensional technique is needed to measure the spatial density of microdamage accumulation. Therefore, the objective of this study was to image microdamage accumulation in cortical bone during fatigue using micro-computed tomography (micro-CT) with a barium sulfate (BaSO4) contrast agent. Bovine cortical bone beams were loaded under four-point bending fatigue. Two symmetric notches were machined on the tensile surface in order to generate damage at the stress concentrations during loading. Specimens were loaded to a specified number of cycles or until one notch fractured, such that the other notch exhibited accumulated microdamage just prior to fracture. Microdamage ahead of the notch was stained by precipitation of BaSO4 and imaged using micro-CT. Reconstructed images showed a distinct region of bright voxels around the notch tip or along propagating cracks due to the presence of BaSO4, which was verified by backscattered electron imaging and energy dispersive spectroscopy. The stained region exhibited a characteristic kidney shape perpendicular to the notch tip, which was correlated to principal strain contours calculated by finite element analysis. The area of stained regions was positively correlated with the number of loading cycles.

Keywords

bonefatiguemicrostructure
Type
Research Article
Information
MRS Online Proceedings Library (OPL) , Volume 898: Symposium L – Mechanical Behavior of Biological and Biomimetic Materials , 2005 , 0898-L09-04
Copyright
Copyright © Materials Research Society 2006

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

REFERENCES

1. Jee, W.S.S., in Bone Mechanics Handbook, edited by Cowin, S. C. (CRC Press, Boca Raton, 2001) pp. 1.1–1.2. Google Scholar
2. Burr, D.B., Forwood, M.R., Fyhrie, D.P., Martin, R.B., Schaffler, M.B. and Turner, C.H., J Bone Miner Res. 12, 6–15 (1997).Google Scholar
3. Martin, R.B., Calcif Tissue Int.73, 101–107 (2003).Google Scholar
4. Schaffler, M.B., Radin, E.L. and Burr, D.B., Bone. 10, 207–214 (1989).Google Scholar
5. Jepsen, K.J. and Davy, D.T., J Biomechanics. 30, 891–894 (1997).Google Scholar
6. Martin, R.B. and Burr, D.B., J Biomechanics. 15, 137–139 (1982).Google Scholar
7. Burr, D.B., Martin, R.B., Schaffler, M.B. and Radin, E.L., J Biomechanics. 18, 189–200 (1985).Google Scholar
8. Mori, S. and Burr, D.B., Bone. 14, 103–109 (1993).Google Scholar
9. Burr, D.B. and Stafford, T., Clin Orthop Rel Res. 260, 305–308 (1990).Google Scholar
10. Lee, T.C., Arthur, T.L., Gibson, L.J. and Hayes, W.C., J Orthop Res. 18, 322–325 (2000).Google Scholar
11. O'Brien, F.J., Taylor, D. and Lee, T.C., J Biomechanics. 35, 523–526 (2002).Google Scholar
12. O'Brien, F.J., Taylor, D. and Lee, T.C., J Biomechanics. 36, 973–980 (2003).Google Scholar
13. Schaffler, M.B., Pitchford, W., Choi, K. and Riddle, J.M., Bone. 15, 483–488 (1994).Google Scholar
14. Zioupos, P. and Currey, J.D., J Biomechanics. 27, 993–5 (1994).Google Scholar
15. O'Brien, F.J., Taylor, D., Dickson, G.R., and Lee, T.C., J Anat. 197, 413–420 (2000).Google Scholar
16. Li, J., Miller, M.A., Hutchins, G.D. and Burr, D.B., Trans Orthop Res Soc. 30, 33 (2005).Google Scholar
17. Leng, H., VanDersarl, J.J., Niebur, G.L. and Roeder, R.K., Trans Orthop Res Soc. 30, 665 (2005).Google Scholar
18. Masse, D.B., Wang, X., Roeder, R.K. and Niebur, G.L., Trans Orthop Res Soc. 30, 35 (2005).Google Scholar
19. Nalla, R.K., Kinney, J.H. and Ritchie, R.O., Nature Mater. 2, 164–168 (2003).Google Scholar
20. Nalla, R.K., Kruzic, J.J., Kinney, J.H. and Ritchie, R.O., Bone. 35, 1240–1246 (2004).Google Scholar
21. Sobelman, O.S., Gibeling, J.C., Stover, S.M., Hazelwood, S.J., Yeh, O.C., Shelton, D.R. and Martin, R.B., J Biomechanics. 37, 1295–1303 (2004).Google Scholar
22. Reilly, D.T. and Burstein, A.H., J Bone Joint Surg. 56, 1001–1022 (1974).Google Scholar
23. Currey, J.D., in Bone Mechanics Handbook, edited by Cowin, S. C. (CRC Press, Boca Raton, 2001) pp. 19.1–19.4. Google Scholar