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Fabrication of Ring-shaped Bioactuator Powered by Cardiomyocytes

Published online by Cambridge University Press:  01 February 2011

Hiroshi Horiguchi ,
Yoshitake Akiyama  and
Keisuke Morishima
Hiroshi Horiguchi
Affiliation:
[email protected], Tokyo University of Agriculture and Technology, Department of Mechanical Systems Engineering, 2-24-16 Nakachou, Koganei, Tokyo, N/A, Japan
Yoshitake Akiyama
Affiliation:
[email protected], Tokyo University of Agriculture and Technology, Department of Mechanical Systems Engineering, 2-24-16 Nakachou, Koganei, Tokyo, 184-8588, Japan
Keisuke Morishima
Affiliation:
[email protected], Tokyo University of Agriculture and Technology, Department of Mechanical Systems Engineering, 2-24-16 Nakachou, Koganei, Tokyo, 184-8588, Japan
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Abstract

We have proposed a novel use of pulsating living cells as a driving source for a micro bio-actuator. Cardiyomyocytes contract autonomously using chemical energy. But, contractile force of a single cardiomyocyte is not enough to actuate a micro robot or a mechanical system. So it is necessary to reconstruct cardiomyocytes to increase the ability to contract. We focused on the reconstruction of cardiomyocytes using a basement membrane protein mixture. In this paper, we fabricated a prototype cardiomyocyte gel structure. We confirmed that the ring-shaped cardiomyocyte gel structure condensed around the central cylinder within the PDMS mold and contracted synchronously and autonomously.

Keywords

microelectro-mechanical (MEMS)biomaterialmicrostructure
Type
Research Article
Information
MRS Online Proceedings Library (OPL) , Volume 1096: Symposium FF – Molecular Motors, Nanomachines and Active Nanostructures , 2008 , 1096-FF03-26
Copyright
Copyright © Materials Research Society 2008

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References

1. Morishima, K., Tanaka, Y., Ebara, M., Shimizu, T., Kikuchi, A., Yamato, M., Okano, T. and Kitamori, T., “Demonstration of a bio-microactuator powered by cultured cardiomyocytes coupled to hydrogel micropillars”, Sensors & Actuators: B.Chemical, 119(1), 345–350, (2006).Google Scholar
2. Tanaka, Y., Morishima, K., Shimizu, T., Kikuchi, A., Yamato, M., Okano, T., Kitamori, T., “An actuated pump on-chip powered by cultured cardiomyocytes”, Lab on a Chip, 6(3), 362–368, (2006).Google Scholar
3. Tanaka, Y., Morishima, K., Shimizu, T., Kikuchi, A., Yamato, M., Okano, T., Kitamori, T., “Demonstration of a PDMS-based bio-microactuator using cultured cardiomyocytes to drive polymer micropillars”, Lab on a Chip, 6(2), 230–235 (2006).Google Scholar
4. Nishimura, S., Yasuda, S., Katoh, M., Yamada, K.P., Yamashita, H., Saeki, Y., Sunagawa, K., Nagai, R., Hisada, T., Sugiura, S., Single cell mechanics of rat cardiomyocytes under isometric, unloaded, and physiologically loaded conditions, Am. J. Physiol. Heart Circulatory Physiol. 287 (2004) H196–H202.Google Scholar
5. Zimmermann, WH., Schneiderbanger, K., Schubert, P., Didié, M., Münzel, F., Heubach, JF., Kostin, S., Neuhuber, WL., Eschenhagen, T., “Tissue Engineering of a Differentiated Cardiac Muscle Construct.”, Circ. Res, 90,223–230(2002)Google Scholar
6. Zimmermann, WH., Didié, M., Wasemeier, GH., Nixdorff, U., Hess, A., Melnychenko, I., Boy, O., Neuhuber, WL., Weyand, M., Eschenhagen, T., “Cardiac grafting of engineered heart tissue in syngenic rats.”, Circulation,102,1151–1157(2002)Google Scholar
7. Zimmermann, WH, Melnychenko, I, Wasmeier, G, Didie, M, Naito, H, Nixdorff, U, Hess, A, Budinsky, L, Brune, K, Michaelis, B, Dhein, S, Schwoerer, A, Ehmke, H, Eschenhagen, T, “Engineered heart tissue grafts improve systolic and diastolic function in infarcted rat hearts.”, Nat. Med, 12,452–45Google Scholar
8. Yamada, N., Okano, T., Sakai, H., Karikusa, F., Sawasaki, Y., Sakurai, Y., “Thermo-responsive polymeric surfaces; control of attachment and detachment of cultured cells,” Makromol. Chem. Rapid Commun., 11, 571–576, (1990).Google Scholar
9. Shimizu, T., Yamato, M., Kikuchi, A., and Okano, T., “Cell sheet engineering for myocardial tissue reconstruction,” Biomaterials, 24, 2309–2316, (2003).Google Scholar