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Experimental study of filling carbon nanotubes with nucleic acids

Published online by Cambridge University Press:  15 March 2011

Daxiang Cui ,
Cengiz S. Ozkan ,
Yong Kong  and
Huajian Gao
Daxiang Cui
Affiliation:
Max Planck Institute for Metals Research, Heisenbergstrasse 3, 70569 Stuttgart, Germany
Cengiz S. Ozkan
Affiliation:
Department of Mechanical Engineering, University of California, Riverside, CA 92521-0425, USA
Yong Kong
Affiliation:
Max Planck Institute for Metals Research, Heisenbergstrasse 3, 70569 Stuttgart, Germany
Huajian Gao
Affiliation:
Max Planck Institute for Metals Research, Heisenbergstrasse 3, 70569 Stuttgart, Germany
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Abstract

Encapsulation of DNA molecules inside carbon nanotubes (CNT) in water were performed under the conditions of 400K and 3Bar. Double stranded DNAs of 2kb and 400bp in length, and single stranded oligonucleotides of 60bp in length were selected as target molecules. Au and Pt nanoparticles and fluorescent dye Cy3 were used as tags. Agarose gel electrophoresis was used to remove DNA molecules attached on the outside walls of CNTs. Laser confocal microscopy, scanning electron microscopy (SEM), high resolution transmission electron microscopy (HR-TEM), energy dispersive x-ray spectroscopy (EDX) were employed to confirm the encapsulation process. The results demonstrated that DNA molecules attached to the outside of CNTs can be removed by electrophoresis. Confocal microscopy and HR-TEM observations as well as EDX analysis confirmed that the Cy3-labelled DNA molecules, Au-labelled oligonucleotides and Pt-labelled DNA fragments can indeed be encapsulated inside CNTs. These experimental results support our earlier molecular dynamics simulations on encapsulating oligonucleotides inside CNTs. The DNA-CNT hybrids could be further explored for potential applications in bio-nanotechnology.

Type
Research Article
Information
MRS Online Proceedings Library (OPL) , Volume 820: Symposia O/R – Nanoengineered Assemblies and Advanced Micro/Nanosystems , 2004 , O4.6
Copyright
Copyright © Materials Research Society 2004

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