Hostname: page-component-cd9895bd7-mkpzs Total loading time: 0 Render date: 2024-12-27T01:54:52.475Z Has data issue: false hasContentIssue false
  • English
  • Français

Experimental investigations into the mechanical properties of the collagen fibril-noncollagenous protein (NCP) interface in antler bone

Published online by Cambridge University Press:  01 February 2011

Fei Hang  and
Asa H Barber
Fei Hang
Affiliation:
[email protected] Queen Mary University of London Centre for Materials Research & School of Engineering and Materials Science, London, United Kingdom
Asa H Barber
Affiliation:
[email protected] Queen Mary University of London Centre for Materials Research & School of Engineering and Materials Science, London, United Kingdom
Get access

Abstract

Antler is an extraordinary bone tissue that displays significant overall toughness when compared to other bone materials. The origin of this toughness is due to the complex interaction between the nanoscale constituents as well as structural hierarchy in the antler material. Of particular interest is the mechanical performance of the interface between the collagen fibrils and considerably smaller volume of non-collagenous protein (NCP) between these fibrils. This paper directly examines the mechanical properties of isolated volumes of antler using combined in situ atomic force microscopy (AFM)-scanning electron microscopy (SEM) experiments. The antler material at the nanoscale is approximated to a fiber reinforced composite, with composite theory used to evaluate the interfacial shear stresses generated between the individual collagen fibrils and NCP during mechanical loading.

Keywords

bonestrengthcomposite
Type
Research Article
Information
MRS Online Proceedings Library (OPL) , Volume 1274: Symposium QQ – Biological Materials and Structures in Physiologically Extreme Conditions and Disease , 2010 , 1274-QQ08-08
Copyright
Copyright © Materials Research Society 2010

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

[1] Currey, J. D. “The design of mineralised hard tissues for their mechanical functions,” Journal of Experimental Biology, vol. 202, pp. 3285–3294, Dec 1999.Google Scholar
[2] Weiner, S. Traub, W. and Wagner, H. D. “Lamellar bone: Structure-function relations,” Journal of Structural Biology, vol. 126, pp. 241–255, Jun 30 1999.Google Scholar
[3] Rho, J. Y. Kuhn-Spearing, L., and Zioupos, P. “Mechanical properties and the hierarchical structure of bone,” Medical Engineering & Physics, vol. 20, pp. 92–102, Mar 1998.Google Scholar
[4] Chen, P. Y. Stokes, A. G. and McKittrick, J. “Comparison of the structure and mechanical properties of bovine femur bone and antler of the North American elk (Cervus elaphus canadensis),” Acta Biomaterialia, vol. 5, pp. 693–706, Feb 2009.Google Scholar
[5] Vashishth, D. Tanner, K. E. and Bonfield, W. “Contribution, development and morphology of microcracking in cortical bone during crack propagation,” Journal of Biomechanics, vol. 33, pp. 1169–1174, Sep 2000.Google Scholar
[6] Gupta, H. S. and Zioupos, P. “Fracture of bone tissue: The ‘hows’ and the ‘whys’,” Medical Engineering & Physics, vol. 30, pp. 1209–1226, Dec 2008.Google Scholar
[7] Tai, K. and Ortiz, C. “Nanomechanical heterogeneity of bone at the length scale of individual collagen fibrils,” Abstracts of Papers of the American Chemical Society, vol. 231, pp. 179-PMSE, Mar 2006.Google Scholar
[8] Nuriel, S. Katz, A. and Wagner, H. D. “Measuring fiber-matrix interfacial adhesion by means of a ‘drag-out’ micromechanical test,” Composites Part a-Applied Science and Manufacturing, vol. 36, pp. 33–37, 2005.Google Scholar
[9] Yue, C. Y. and Cheung, W. L. “Interfacial Properties of Fiberreinforced Composites,” Journal of Materials Science, vol. 27, pp. 3843–3855, Jul 1992.Google Scholar
[10] Barber, A. H. Cohen, S. R. Kenig, S. and Wagner, H. D. “Interfacial fracture energy measurements for multi-walled carbon nanotubes pulled from a polymer matrix,” Composites Science and Technology, vol. 64, pp. 2283–2289, Nov 2004.Google Scholar
[11] Hsueh, C. H. “Interfacial Debonding and Fiber Pull-Out Stresses of Fiber-Reinforced Composites. 6. Interpretation of Fiber Pull-Out Curves,” Materials Science and Engineering a-Structural Materials Properties Microstructure and Processing, vol. 149, pp. 11–18, Dec 1991.Google Scholar
[12] Kim, J. K. Baillie, C. and Mai, Y. W. “Interfacial Debonding and Fiber Pull-Out Stresses.1. Critical Comparison of Existing Theories with Experiments,” Journal of Materials Science, vol. 27, pp. 3143–3154, Jun 1992.Google Scholar
[13] Barber, A. H. Cohen, S. R. Eitan, A. Schadler, L. S. and Wagner, H. D. “Fracture transitions at a carbon-nanotube/polymer interface,” Advanced Materials, vol. 18, pp. 83–87, Jan 2006.Google Scholar
[14] Latour, R. A. Black, J. and Miller, B. “Fracture Mechanisms of the Fiber Matrix Interfacial Bond in Fiber-Reinforced Polymer Composites,” Surface and Interface Analysis, vol. 17, pp. 477–484, Jun 1991.Google Scholar
[15] Gutsmann, T. Hassenkam, T. Cutroni, J. A. and Hansma, P. K. “Sacrificial bonds in polymer brushes from rat tail tendon functioning as nanoscale velcro,” Biophysical Journal, vol. 89, pp. 536–542, Jul 2005.Google Scholar
[16] Graham, J. S. Vomund, A. N. Phillips, C. L. and Grandbois, M. “Structural changes in human type I collagen fibrils investigated by force spectroscopy,” Experimental Cell Research, vol. 299, pp. 335–342, Oct 2004.Google Scholar
[17] Rijt, J. A. J. van der, Werf, K. O. van der, Bennink, M. L. Dijkstra, P. J. and Feijen, J. “Micromechanical testing of individual collagen fibrils,” Macromolecular Bioscience, vol. 6, pp. 697–702, Sep 2006.Google Scholar
[18] Hang, F. Lu, D. and Barber, A. H. “Combined AFM-SEM for mechanical testing of fibrous biological materials,” in Structure-Property Relationships in Biomineralized and Biomimetic Composites. vol. 1187, Kisailus, D. Estroff, L. Gupta, H. S. landis, W. J. and Zavattieri, P. D., Eds. Warrendale: Materials Research Society, 2009, pp. 135–140.Google Scholar
[19] Currey, J. D. Brear, K. and Zioupos, P. “Dependence of Mechanical-Properties on Fiber Angle in Narwhal Tusk, a Highly Oriented Biological Composite,” Journal of Biomechanics, vol. 27, pp. 885-&, Jul 1994.Google Scholar
[20] Vashishth, D. Behiri, J. C. and Bonfield, W. “Crack growth resistance in cortical bone: Concept of microcrack toughening,” Journal of Biomechanics, vol. 30, pp. 763–769, Aug 1997.Google Scholar
[21] Zioupos, P. Wang, X. T. and Currey, J. D. “The accumulation of fatigue microdamage in human cortical bone of two different ages in vitro,” Clinical Biomechanics, vol. 11, pp. 365–375, Oct 1996.Google Scholar