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Evaluation of Cytokine Delivery Systems for Cancer Immunotherapy

Published online by Cambridge University Press:  15 February 2011

Y.S. Jong ,
N.K. Egilmez ,
F-A Chen ,
J.S. Jacob ,
L.P. Smith ,
T.S. Mottl ,
R.B. Bankert  and
E. Mathiowitz
Y.S. Jong
Affiliation:
Artificial Organs Laboratory, Brown University, Providence RI 02912
N.K. Egilmez
Affiliation:
Molecular Immunology, Roswell Park Cancer Institute, Buffalo NY 14263
J.S. Jacob
Affiliation:
Artificial Organs Laboratory, Brown University, Providence RI 02912
L.P. Smith
Affiliation:
Artificial Organs Laboratory, Brown University, Providence RI 02912
T.S. Mottl
Affiliation:
Artificial Organs Laboratory, Brown University, Providence RI 02912
R.B. Bankert
Affiliation:
Molecular Immunology, Roswell Park Cancer Institute, Buffalo NY 14263
E. Mathiowitz
Affiliation:
Artificial Organs Laboratory, Brown University, Providence RI 02912
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Abstract

Cytokines are ideal candidates for controlled release systems; they typically have short half-lives in vivo and may be more effective with localized delivery. A novel microencapsulation technique, phase inversion nanoencapsulation (PIN), was used to encapsulate interleukin-2 or interleukin-12 in biodegradable polymer microspheres. Cytokine was quantified by ELISA and bioactivity analyzed using a murine cytotoxic Tcell line proliferation assay. The release rates of IL-2 was dependant on release buffer components; inclusion of 10% fetal calf serum resulted in significantly higher release rates compared to plain buffer. Encapsulated IL-2 remained stable at all timepoints assayed (up to 4 months) when stored at 4 °C. Results indicated that PIN can be used to encapsulate IL-2 and IL-12, which were released throughout the duration of the release study (up to one month). These microsphere formulations have shown tumor suppressive affects in vivo and may provide a viable alternative to gene transfer for cancer immunotherapy.

Type
Research Article
Information
MRS Online Proceedings Library (OPL) , Volume 550: Symposium GG/HH/II – Biomedical Materials-Drug Delivery, Implants and Tissue Engr , 1998 , 71
Copyright
Copyright © Materials Research Society 1999

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