Hostname: page-component-cd9895bd7-gvvz8 Total loading time: 0 Render date: 2024-12-27T01:55:41.506Z Has data issue: false hasContentIssue false
  • English
  • Français

Enhanced Detection of Cardiovascular Biomarker Proteins: A Detailed Study of Nanoconfinement in Nanoporous Membrane

Published online by Cambridge University Press:  19 March 2012

Savindra Brandigampala ,
Paige Feikert ,
Krishna Vattipalli  and
Shalini Prasad
Savindra Brandigampala
Affiliation:
Department of Electrical Engineering and Computer Science, Wichita State University, 1845 Fairmount St, Wichita, KS 67260, U.S.A.
Paige Feikert
Affiliation:
Bio-Engineering Program, College of Engineering, Wichita State University, 1845 Fairmount St, Wichita, KS 67260, U.S.A.
Krishna Vattipalli
Affiliation:
Department of Electrical Engineering and Computer Science, Wichita State University, 1845 Fairmount St, Wichita, KS 67260, U.S.A.
Shalini Prasad
Affiliation:
Department of Electrical Engineering and Computer Science, Wichita State University, 1845 Fairmount St, Wichita, KS 67260, U.S.A. Bio-Engineering Program, College of Engineering, Wichita State University, 1845 Fairmount St, Wichita, KS 67260, U.S.A.
Get access

Abstract

The goal of this work is to understand the role of nano-confinement in designing an inexpensive and user friendly ‘point- of- care’ (POC) protein biosensor. We used printed circuit board based gold chips and integrated them with nanoporous alumina membranes in generating high density arrays of nano scale confined spaces. We initially tested the role of a nanomembrane in achieving signal enhancement through size based confinement of proteins. As a later part of the experiment, we studied the role of pore size on achieving signal enhancement by using membranes of two different pore sizes of 100 and 200 nm. It is critical that ultralow detection of biomolecules be achieved as they have significant impact in designing diagnostics platforms for early disease diagnosis. Commercially available nano-porous membranes made out of anodized alumina were evaluated for their role in nano-confinement and enhancing sensitivity of detection. In this biosensor configuration sandwich assay, an electrical double layer is formed between a test protein (C-reactive protein) and the gold surface underneath the porous membrane. Using electrical impedance spectroscopy, the capacitance/impedance changes in the electrical double layer, was analyzed which translated to identifying the sensitivity and the linear dose response of the sensor for two specific conditions (a) with nano confinement and (b) for varying size of confined spaces

Keywords

nanostructuresensorbiomedical
Type
Articles
Information
MRS Online Proceedings Library (OPL) , Volume 1355: Symposium JJ – Biological Hybrid Materials for Life Sciences , 2011 , mrss12-1355-jj09-15
Copyright
Copyright © Materials Research Society 2012

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

REFERENCES

1. Wang, J.. “Nanomaterial-based electrochemical biosensors”. Analyst, 130(4):421–426, 2005.10.1039/b414248aGoogle Scholar
2. Stoeva, S.I., Lee, J.S., Smith, J.E., Rosen, S.T., and Mirkin, C.A.. “Multiplexed detection of protein cancer markers with biobarcoded nanoparticle probes”. J. Am. Chem. Soc, 128(26):8378–8379, 2006.10.1021/ja0613106Google Scholar
3. Dequaire, M., Degrand, C., and Limoges, B.. “An electrochemical metalloimmunoassay based on a colloidal gold label”. Anal. Chem, 72(22):5521–5528, 2000.10.1021/ac000781mGoogle Scholar
4. Cui, Y. and Lieber, C.M.. “Functional Nanoscale Electronic Devices Assembled Using Silicon Nanowire Building Blocks”, 2001.Google Scholar
5. Desai, T.A., Hansford, D.J., Kulinsky, L., Nashat, A.H., Rasi, G., Tu, J., Wang, Y., Zhang, M., and Ferrari, M.. “Nanopore Technology for Biomedical Applications”. Biomedical Microdevices, 2(1):11–40, 1999.10.1023/A:1009903215959Google Scholar
6. Lee, S.B., Mitchell, D.T., Tron, L., Nevanen, T.K., Soderlund, H., and Martin, C.R.. “Antibody-Based Bio-Nanotube Membranes for Enantiomeric Drug Separations”. 2002.Google Scholar
7. Zimmerman, SB and Minton, AP. “Macromolecular crowding: biochemical, biophysical, and physiological consequences”. Annu Rev Biophys Biomol Struct, 22:27–65, 1993.10.1146/annurev.bb.22.060193.000331Google Scholar
8. Ellis, R.J.. “Macromolecular crowding: obvious but underappreciated”. Trends Biochem. Sci, 26(10):597–604, 2001.10.1016/S0968-0004(01)01938-7Google Scholar
9. Ridker, Paul M. “High-sensitivity creactive protein, inflammation, and cardiovascular risk: from concept to clinical practice to clinical benefit”. Am Heart J, 148(1 Suppl):S19–26, 2004.10.1016/j.ahj.2004.04.028Google Scholar
10. Reddy, Ravikiran K., Bothara, Manish G., Barrett, Thomas W., Carruthers, John, and Prasad, Shalini. “Nanomonitors: Protein Biosensors for Rapid Analyte Analysis”, IEEE SENSORS JOURNAL, VOL. 8, NO. 6, 2008.10.1109/JSEN.2008.923177Google Scholar
11. Nagaraj, Vinay J., Aithal, Srivatsa, Eaton, Seron, Bothara, Manish, Wiktor, Peter, and Prasad, Shalini, “NanoMonitor: a miniature electronic biosensor for glycan biomarker detection”, Nanomedicine, 5(3), 369–378, 2010.10.2217/nnm.10.11Google Scholar
12. Bothara, M., Venkataraman, V., Reddy, R. K. K., Barrett, T., Carruthers, J. and Prasad, S, “Nanomonitors: Electrical immunoassays for protein biomarker profiling”, Nanomedicine, 3(4), 423–436, 2010.10.2217/17435889.3.4.423Google Scholar