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Electrochemical Release of Immobilized IgG Protein

Published online by Cambridge University Press:  01 February 2011

Tanveer Mahmud ,
Wojtek Wlodarski ,
Arnan Mitchell ,
Sally Gras ,
Adrian Trinchi  and
Kourosh Kalantar-zadeh
Tanveer Mahmud
Affiliation:
[email protected], RMIT University, School of Electrical and Computer Engineering, Melbourne, 3001, Australia
Wojtek Wlodarski
Affiliation:
[email protected], RMIT University, School of Electrical and Computer Engineering, Melbourne, 3001, Australia
Arnan Mitchell
Affiliation:
[email protected], RMIT University, School of Electrical and Computer Engineering, Melbourne, 3001, Australia
Sally Gras
Affiliation:
[email protected], The University of Melbourne, Department of Chemical and Biomolecular Engineering, and The Bio21 Molecular Science & Biotechnology, Melbourne, 3004, Australia
Adrian Trinchi
Affiliation:
[email protected], CSIRO, Manufacturing and Materials Technology, Melbourne, N/A, Australia
Kourosh Kalantar-zadeh
Affiliation:
[email protected], RMIT University, School of Electrical and Computer Engineering, Melbourne, 3001, Australia
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Abstract

In this paper, we present the electrochemically programmed release of immobilized IgG protein molecules that have been attached to gold coated surfaces via a thiol-gold linkage. Fluorescence microscopy has been used to image the release of fluorescently tagged IgGs in phosphate buffered saline. In this technique, the reductive desorption of self-assembled monolayers is employed for the release of proteins, which are immobilized on the surface either by non-covalent or covalent interactions. The voltage applied for the release of proteins is in a range of -1.5V to -60V.

Keywords

surface chemistrybiologicalself-assembly
Type
Research Article
Information
MRS Online Proceedings Library (OPL) , Volume 1010: Symposium V – Functional Materials for Chemical and Biochemical Sensors , 2007 , 1010-V06-01
Copyright
Copyright © Materials Research Society 2007

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References

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