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Efficacy of Tobramycin Conjugated to Superparamagnetic Iron Oxide Nanoparticles in Treating Cystic Fibrosis Infections

Published online by Cambridge University Press:  19 November 2013

Marek Osiński ,
Yekaterina I. Brandt ,
Leisha M. Armijo ,
Michael Kopciuch ,
Nathan. J. Withers ,
Nathaniel C. Cook ,
Natalie L. Adolphi ,
Gennady A. Smolyakov  and
Hugh D. C. Smyth
Marek Osiński
Affiliation:
Center for High Technology Materials, University of New Mexico, 1313 Goddard SE, Albuquerque, NM 87106-4343, U.S.A. Tel. +1 (505) 272-7812; Fax +1 (505) 272-7801; E-mail: [email protected]
Yekaterina I. Brandt
Affiliation:
Center for High Technology Materials, University of New Mexico, 1313 Goddard SE, Albuquerque, NM 87106-4343, U.S.A. Tel. +1 (505) 272-7812; Fax +1 (505) 272-7801; E-mail: [email protected]
Leisha M. Armijo
Affiliation:
Center for High Technology Materials, University of New Mexico, 1313 Goddard SE, Albuquerque, NM 87106-4343, U.S.A. Tel. +1 (505) 272-7812; Fax +1 (505) 272-7801; E-mail: [email protected]
Michael Kopciuch
Affiliation:
Center for High Technology Materials, University of New Mexico, 1313 Goddard SE, Albuquerque, NM 87106-4343, U.S.A. Tel. +1 (505) 272-7812; Fax +1 (505) 272-7801; E-mail: [email protected]
Nathan. J. Withers
Affiliation:
Center for High Technology Materials, University of New Mexico, 1313 Goddard SE, Albuquerque, NM 87106-4343, U.S.A. Tel. +1 (505) 272-7812; Fax +1 (505) 272-7801; E-mail: [email protected]
Nathaniel C. Cook
Affiliation:
Center for High Technology Materials, University of New Mexico, 1313 Goddard SE, Albuquerque, NM 87106-4343, U.S.A. Tel. +1 (505) 272-7812; Fax +1 (505) 272-7801; E-mail: [email protected]
Natalie L. Adolphi
Affiliation:
Department of Biochemistry and Molecular Biology, School of Medicine, University of New Mexico, Albuquerque, NM 87131, U.S.A.
Gennady A. Smolyakov
Affiliation:
Center for High Technology Materials, University of New Mexico, 1313 Goddard SE, Albuquerque, NM 87106-4343, U.S.A. Tel. +1 (505) 272-7812; Fax +1 (505) 272-7801; E-mail: [email protected]
Hugh D. C. Smyth
Affiliation:
College of Pharmacy, University of Texas at Austin, Austin, TX 78712, U.S.A.
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Abstract

Cystic fibrosis (CF) is an inherited childhood-onset life-shortening disease. It is characterized by increased respiratory production, leading to airway obstruction, chronic lung infection and inflammatory reactions. The most common bacteria causing persisting infections in people with CF is Pseudomonas aeruginosa. Superparamagnetic Fe3O4 iron oxide nanoparticles (NPs) conjugated to the antibiotic (tobramycin), guided by a gradient of the magnetic field or subjected to an oscillating magnetic field, show promise in improving the drug delivery across the mucus and P. aeruginosa biofilm to the bacteria. The question remains whether tobramycin needs to be released from the NPs after the penetration of the mucus barrier in order to act upon the pathogenic bacteria. We used a zero-length 1-ethyl-3-[3-dimethylaminopropyl] carbodiimide hydrochloride (EDC) crosslinking agent to couple tobramycin, via its amine groups, to the carboxyl groups on Fe3O4 NPs capped with citric acid. The therapeutic efficiency of Fe3O4 NPs attached to the drug versus that of the free drug was investigated in P. aeruginosa culture.

Keywords

colloidalIron OxideNanoparticles
Type
Articles
Information
MRS Online Proceedings Library (OPL) , Volume 1617: Symposium 7E – Low-Dimensional Semiconductor Structures , 2013 , pp. 127 - 137
Copyright
Copyright © Materials Research Society 2013 

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References

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