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Dextran Based Polyampholyte Having Cryoprotective Properties

Published online by Cambridge University Press:  15 February 2013

Minkle Jain  and
Kazuaki Matsumura
Minkle Jain
Affiliation:
School of Materials Science, Japan Advanced Institute of Science and Technology, 1-1 Asahidai, Nomi, Ishikawa 923-1292 Japan M. Tech (CSPT), Department of Chemistry, University of Delhi, Delhi-110007, India
Kazuaki Matsumura
Affiliation:
School of Materials Science, Japan Advanced Institute of Science and Technology, 1-1 Asahidai, Nomi, Ishikawa 923-1292 Japan
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Abstract

Dimethyl sulfoxide (DMSO) has been used for several decades as the most efficient cryoprotective agent (CPA) for many types of cells in spite of its cytotoxicity and its effect on differentiation. Recently we showed that carboxylated poly-L-lysine, which is classified as a polyampholyte, has a cryoprotective effect on cells in solution without any other cryoprotectant. Here we developed high molecular weight polyampholytes with an appropriate ratio of amino and carboxyl groups and evaluated their cryopreservation efficiency. A novel polyampholyte based on naturally available polymer dextran, in which we introduced both amino and carboxyl groups shows an excellent post thaw-survival efficiency of more than 90% of murine L929 cells. It can serve as the sole high molecular weight CPA for tissue engineering applications without animal derived materials.

Keywords

biomaterialpolymerbiomedical
Type
Articles
Information
MRS Online Proceedings Library (OPL) , Volume 1498: Symposium L – Biomimetic, Bio-inspired and Self-Assembled Materials for Engineered Surfaces and Applications , 2013 , pp. 33 - 38
Copyright
Copyright © Materials Research Society 2013 

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References

REFERENCES

Karlsson, J.O.M., Toner, M., Biomaterials, 17, 243–256 (1996).CrossRefGoogle Scholar
Karow, A.M. Jr. and Webb, W.R., Cryobiology 1, 270–273(1965).CrossRefGoogle Scholar
Karow, A.M. Jr., J Pharm Pharmacol, 21, 209–223(1969).CrossRefGoogle Scholar
Fahy, G.M., Cryobiology, 23, 1–13 (1986).CrossRefGoogle ScholarPubMed
Oh, J.E., Karlmark Raja, K., Shin, J.H., Pollak, A., Hengstschlager, M., Lubec, G., Amino Acids, 31, 289–98 (2006).CrossRefGoogle Scholar
Young, D.A., Gavrilov, S., Pennington, C.J., Nuttall, R.K., Edwards, D.R., Kitsis, R.N. et al. ., Biochem. Biophys. Res. Commun., 322, 759–65 (2004).CrossRefGoogle Scholar
Matsumura, K., Hyon, S.H., Biomaterials 30, 4842–4849 (2009).CrossRefGoogle Scholar
Matsumura, K., Bae, J.Y., Hyon, S.H., Cell Transplant. 19, 691–699 (2010).CrossRefGoogle Scholar
Habeeb, A.F., Anal Biochem, 14, 328–36 (1966).CrossRefGoogle Scholar
Mazur, P., Cryobiology, 14, 251–72 (1977).CrossRefGoogle Scholar
Mazur, P., Pinn, I.L., Kleinhans, F.W., Cryobiology, 5, 158–66 (2007).CrossRefGoogle Scholar
Toner, M., Cravalho, E.G., Stachecki, J., Fitzgerald, T., Tompkins, R.G., Yarmush, M.L. et al. ., Biophys J, 64, 1908–21 (1993).CrossRefGoogle Scholar