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The Development of an In Situ Assay for bFGF Release

Published online by Cambridge University Press:  15 February 2011

S. K. Hobbs ,
L. M. Periolat ,
L. G. Cima ,
M. Nugent ,
M. Leunig  and
R. K. Jain
S. K. Hobbs
Affiliation:
Department of Chemical Engineering, Massachusetts Institute of Technology, Cambridge, MA 02139
L. M. Periolat
Affiliation:
Department of Chemical Engineering, Massachusetts Institute of Technology, Cambridge, MA 02139
L. G. Cima
Affiliation:
Department of Chemical Engineering, Massachusetts Institute of Technology, Cambridge, MA 02139
M. Nugent
Affiliation:
Boston University School of Medicine, Boston MA 02118
M. Leunig
Affiliation:
Boston University School of Medicine, Boston MA 02118
R. K. Jain
Affiliation:
Boston University School of Medicine, Boston MA 02118
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Abstract

There is a need for an in situ assay to quantify tissue reactivity to sustained release of bFGF to better understand and control growth factor-induced angiogenesis. To this end we have adapted the alginate/heparin-sepharose release system for use in the mouse dorsal skinfold chamber. A mathematical model was used to predict the time dependence of bFGF release as a function of bFGF loading, heparin concentration, and device geometry. The model predictions agreed well with previously reported in vitro data. In vivo studies to correlate blood vessel growth as a function of release rate are in progress.

Type
Research Article
Information
MRS Online Proceedings Library (OPL) , Volume 331: Symposium T – Biomaterials for Drug and Cell Delivery , 1993 , 187
Copyright
Copyright © Materials Research Society 1994

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